上海博研生物工程研究中心竭誠為您提供的產(chǎn)品和服務(wù)，期待您的來電,Elisa試劑盒，Elisa試劑盒說明書，Elisa試劑盒價格
產(chǎn)品名稱：豬白介素1受體拮抗劑（IL1Ra）ELISA試劑盒服務(wù)專業(yè)
英文名稱：PoFAine Interleukin 1 receptor antagonist, IL-1ra ElisaKit.
產(chǎn)品編號：BYS12578B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實(shí)驗(yàn)
產(chǎn)品規(guī)格：96T/48T
ELISA實(shí)驗(yàn)原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標(biāo)本或者標(biāo)準(zhǔn)品、*化的抗體、HRP-標(biāo)記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色， 并在酸的作用下轉(zhuǎn)化成zui終的黃色。 顏色的深淺和樣品中的目標(biāo)蛋白呈正相關(guān)。用酶標(biāo)儀在（450nm）波長下測定測定O.D.值（吸光度），計(jì)算樣品濃度。.
豬白介素1受體拮抗劑（IL1Ra）ELISA試劑盒服務(wù)專業(yè) 相關(guān)產(chǎn)品：
豬補(bǔ)體蛋白3（C3）試劑盒說明書，96T/48T*
豬組胺（HIS）試劑盒說明書，96T/48T*
豬轉(zhuǎn)化生長因子β1（TGF-β1）試劑盒說明書，96T/48T*
豬腫瘤壞死因子α（TNF-α）試劑盒說明書，96T/48T*
豬脂聯(lián)素（ADP）試劑盒說明書，96T/48T*
豬載脂蛋白B100（apo-B100）試劑盒說明書，96T/48T*
豬載脂蛋白A1（apo-A1）試劑盒說明書，96T/48T*
豬胰島素樣生長因子結(jié)合蛋白3（IGFBP-3）試劑盒說明書，96T/48T*
豬胰島素樣生長因子2（IGF-2）試劑盒說明書，96T/48T*
豬胰島素樣生長因子1（IGF-1）試劑盒說明書，96T/48T*
人抗鈣蛋白酶抑素抗體（ACAST-DⅣ）試劑盒說明書，96T/48T*
人抗神經(jīng)母細(xì)胞瘤抗體（NB-Ab）試劑盒說明書，96T/48T*
人α1微球蛋白（α1-MG）試劑盒說明書，96T/48T*
人內(nèi)臟脂肪特異性*蛋白酶抑制劑（vaspin）試劑盒說明書，96T/48T*
人過氧化物酶體增殖物激活受體α（PPAR-α）試劑盒說明書，96T/48T*
人系統(tǒng)性紅斑狼瘡（SLE）試劑盒說明書，96T/48T*
人抗*抗體（GM1）試劑盒說明書，96T/48T*
人真胰島素（TI）試劑盒說明書，96T/48T*
人抗中性粒細(xì)胞顆粒抗體（ANGA）試劑盒說明書，96T/48T*
人抗中性粒細(xì)胞抗體（ANA）試劑盒說明書，96T/48T*
The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve
