上海博研生物工程研究中心竭誠為您提供的產(chǎn)品和服務，期待您的來電,Elisa試劑盒，Elisa試劑盒說明書，Elisa試劑盒價格

產(chǎn)品名稱：豬單核細胞增多性李斯特菌素（listeriolysin）ELISA試劑盒極力*    
英文名稱：PoFAine listeriolysin  ElisaKit.
產(chǎn)品編號：BYS12633B
產(chǎn)品性狀：液體
產(chǎn)品用途：科研實驗
產(chǎn)品規(guī)格：96T/48T

ELISA實驗原理: 用純化的抗體包被微孔板，制成固相載體，往包被抗體的微孔中依次加入標本或者標準品、*化的抗體、HRP-標記的親和素，經(jīng)過*洗滌后用底物顯色。底物在過氧化物酶的催化下轉化成藍色， 并在酸的作用下轉化成zui終的黃色。 顏色的深淺和樣品中的目標蛋白呈正相關。用酶標儀在（450nm）波長下測定測定O.D.值（吸光度），計算樣品濃度。.

豬單核細胞增多性李斯特菌素（listeriolysin）ELISA試劑盒極力*  相關產(chǎn)品：

人胱天蛋白酶9（Casp-9）試劑盒說明書，96T/48T*
人中性粒細胞彈性蛋白酶（NE）試劑盒說明書，96T/48T*
人抗肌內膜抗體IgA（EMA IgA）試劑盒說明書，96T/48T*
人腎損傷分子1（Kim-1）試劑盒說明書，96T/48T*
人抗糖蛋白抗體（GP）試劑盒說明書，96T/48T*
人抗單核細胞抗體（AMA）試劑盒說明書，96T/48T*
人氧化低密度脂蛋白抗體（OLAb）試劑盒說明書，96T/48T*
人髓過氧化物酶（MPO）試劑盒說明書，96T/48T*
人末端補體復合物C5b-9（TCC C5b-9）試劑盒說明書，96T/48T*
人補體蛋白4（C4）試劑盒說明書，96T/48T*
兔子巨噬細胞炎性蛋白1α（MIP-1α/CCL3）試劑盒說明書，96T/48T*
兔子神經(jīng)膠質纖維酸性蛋白（GFAP）試劑盒說明書，96T/48T*
豚鼠白介素4（IL-4）試劑盒說明書，96T/48T*
豚鼠免疫球蛋白G（IgG）試劑盒說明書，96T/48T*
豚鼠血清總補體（CH50）試劑盒說明書，96T/48T*
豚鼠組胺（HIS）試劑盒說明書，96T/48T*
豚鼠轉化生長因子β1（TGF-β1）試劑盒說明書，96T/48T*
豚鼠腫瘤壞死因子α（TNF-α）試劑盒說明書，96T/48T*
豚鼠生長激素（GH）試劑盒說明書，96T/48T*
豚鼠內皮素1（ET-1）試劑盒說明書，96T/48T*

The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve