瘦素受體重疊轉(zhuǎn)錄基因1抗體，Anti-LEPROTL1抗體簡單介紹：
產(chǎn)品編號：BYK-18234R
英文名稱：Anti-LEPROTL1
中文名稱：瘦素受體重疊轉(zhuǎn)錄基因1抗體
別名：HSPC112; Leprotl1; Leptin receptor overlapping transcript-like 1; LERL1_HUMAN; my047; Vps55
背景介紹：Mutation of Ob (Obesity factor), also known as leptin precursor, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. The Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor. LEPROTL1 (Leptin receptor overlapping transcript-like 1) is a 131 amino acid multi-pass membrane protein that is highly homologous to the leptin receptor gene-related protein, Ob-R. LEPROTL1 is widely expressed and contains a JAK binding site. The LEPROTL1 gene is located on chromosome 8, which is made up of nearly 146 million bases and encodes about 800 genes.
產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*
抗體來源 Rabbit or Mouse
克隆類型 Polyclonal or Monoclonal
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性 狀 Lyophilized or Liquid
濃 度 1mg/ml
瘦素受體重疊轉(zhuǎn)錄基因1抗體，Anti-LEPROTL1抗體產(chǎn)品信息：
冰凍切片的免疫組化染色步驟
- 速凍組織
- 冰凍切片4~8μm，冰凍切片后如不染色，必須吹干，儲存低溫冰箱內(nèi)，或進行短暫預(yù)固定后儲存于-20℃冰箱
- 室溫放置30分鐘后，入4℃丙酮固定10分鐘，也可根據(jù)需要選擇其他的固定方式
- PBS沖洗，5分鐘×3次
- 用3%過氧化氫孵育5~10分鐘，消除內(nèi)源性過氧化物酶的活性
- PBS沖洗，5分鐘×3次
- 下接石蠟切片免疫組化染色操作步驟（滴加一抗開始）
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