德國cellast單細(xì)胞力學(xué)加載儀-生命科學(xué)儀器-儀表網(wǎng)

公司介紹主營產(chǎn)品

專注力學(xué)生物學(xué)、3D細(xì)胞生物打印科研儀器的代理銷售、?租賃、實驗技術(shù)培訓(xùn),做中國較好細(xì)胞力學(xué)、3D細(xì)胞打印科研利器提供商與技術(shù)服務(wù)商。企業(yè)的主要目標(biāo)是：把歐美日韓等國家的（細(xì)胞生物分子力學(xué)）力學(xué)生物和3D細(xì)胞生物打印前沿產(chǎn)品與技術(shù)引進中國，提供較好的細(xì)胞力學(xué)、3D細(xì)胞打印實驗技術(shù)培訓(xùn)與設(shè)備租賃，讓國內(nèi)科研單位緊跟或科研發(fā)展的較早動向。為客戶提供高效經(jīng)濟的解決方案。

單分子操縱磁鑷裝置，單細(xì)胞力學(xué)加載儀，細(xì)胞組織應(yīng)力加載培養(yǎng)系統(tǒng)，3D細(xì)胞灌流培養(yǎng)系統(tǒng)，皮膚、傷口生物械力特性實時測試分析系統(tǒng)，Aurora Scientific肌肉力學(xué)測試系統(tǒng)，神經(jīng)外科創(chuàng)傷性腦損傷模型系統(tǒng)，Cellexus高效內(nèi)環(huán)流氣升式生物反應(yīng)器，Moxi Z細(xì)胞計數(shù)儀,Moxiflow便攜式流式細(xì)胞儀，顯微鏡載物臺CO2培養(yǎng)箱籠式CO2顯微鏡培養(yǎng)箱，便攜式細(xì)胞/微生物氧環(huán)境控制培養(yǎng)系統(tǒng)，模塊化多通道三維灌流培養(yǎng)系統(tǒng)，Piuma 納米壓痕測試力學(xué)分析儀，瑞士regenhu3d生物打印機，全自動、高精準(zhǔn)單細(xì)胞捕獲操縱、沉積系統(tǒng)，德國bioram單細(xì)胞拉曼光譜無損測定鑒別系統(tǒng)

點擊展示更多內(nèi)容

產(chǎn)品簡介在線詢價

適用領(lǐng)域	科研

該單細(xì)胞高通量牽張與力學(xué)特性測試分析系統(tǒng),是世界臺用來高通量測量、分析單個懸浮細(xì)胞形變的設(shè)備。用來可視化研究討論細(xì)胞力學(xué)性質(zhì)與其功能之間的關(guān)系該系統(tǒng)可以安裝在任何相位差顯微鏡上的模塊。溫度穩(wěn)定和激光安全。

德國cellast單細(xì)胞力學(xué)加載儀-生命科學(xué)儀器產(chǎn)品詳情

單細(xì)胞力學(xué)加載、高通量單個細(xì)胞應(yīng)力加載刺激、高通量單個細(xì)胞流變、單細(xì)胞應(yīng)力應(yīng)變系統(tǒng)

單細(xì)胞牽張拉伸壓縮形變與機械力特性測試分析系統(tǒng)

——基于微流控技術(shù)的單細(xì)胞機械力特性精準(zhǔn)、高通量表征
Single cell mechanics - the easy way

背景：
單細(xì)胞水平的機械力特性表征，可以有效闡明細(xì)胞的功能和狀態(tài)，揭示細(xì)胞的單體差異性，對于細(xì)胞的分化和病理研究，以及疾病的早期臨床診斷和治療具有非常重要的意義。該系統(tǒng)基于微流控芯片的方式更適合單細(xì)胞樣本的微環(huán)境精確控制、高通量定向操縱及多參數(shù)非特異性檢測。
簡介：
該單細(xì)胞高通量牽張與力學(xué)特性測試分析系統(tǒng),是世界臺用來高通量測量、分析單個懸浮細(xì)胞形變的設(shè)備。用來可視化研究討論細(xì)胞力學(xué)性質(zhì)與其功能之間的關(guān)系該系統(tǒng)可以安裝在任何相位差顯微鏡上的模塊。溫度穩(wěn)定和激光安全。
單細(xì)胞牽張拉伸壓縮形變與機械力特性測試分析系統(tǒng)

單個細(xì)胞加力模式如下圖：
由兩束細(xì)胞無損激光夾持細(xì)胞進行牽拉或擠壓，進而使單個細(xì)胞受到牽張拉伸或壓縮力刺激。

系統(tǒng)亮點特性：

1 ）可大量表征單細(xì)胞機械力特性、操作簡便、樣品消耗量小
該系統(tǒng)的微流控芯片具有與細(xì)胞直徑良好相符性的微納米級腔道，并能實現(xiàn)對微流體的精確控制，使其尤其適合單細(xì)胞機械特性研究分析，該微流控的高通量技術(shù)便于大量表征單細(xì)胞機械力特性、操作簡便、樣品消耗量小、集成和微型化程度高等優(yōu)點，且在分析過程中單細(xì)胞懸浮高速流經(jīng)檢測區(qū)域，該連續(xù)流動態(tài)檢測的特性*提高了系統(tǒng)的通量。
2）高速對單個細(xì)胞進行形變，并進行機械特性高速表征，單細(xì)胞高通量流變
利用兩素未聚焦光進行單細(xì)胞形變，并通過圖形化微柱基地表征細(xì)胞的力特性,高速有效分析單細(xì)胞水平的機械特性, 高達(dá)300個細(xì)胞/小時.
3）非機械接觸、無標(biāo)記進行細(xì)胞捕捉和拉伸，確保細(xì)胞安全與細(xì)胞損傷***小化(Contact-free cell deformation)
利用光延伸器技術(shù)測試細(xì)胞機械特性能時，在非機械接觸情況下細(xì)胞進行捕捉和拉伸，且不需要對激光進行聚焦，能實現(xiàn)細(xì)胞損傷***小化。優(yōu)于AFM（原子力顯微鏡）和光鑷
4）將光延伸器安全性與微流控高通量*相結(jié)合，細(xì)胞機械特性測試分析安全而且高效
采用2個微流道來輸送細(xì)胞，使兩條光纖垂直分布于通道兩側(cè)并嚴(yán)格對準(zhǔn)? ，單細(xì)胞隨流體進入檢測區(qū)域時，首先采用功率較低的光速捕獲細(xì)胞，然后增加光速的功率使細(xì)胞發(fā)生形變。通過對細(xì)胞變形能力的分析，不僅能區(qū)分病變細(xì)胞和正常細(xì)胞，而且可以用于辨別轉(zhuǎn)型特性和非轉(zhuǎn)移特性的癌細(xì)胞。
5)自動化測量單細(xì)胞力屬性和成像記錄細(xì)胞形變記錄
對應(yīng)于用戶定義的拉伸模式，細(xì)胞被自動傳送到測量區(qū)域由CellStretcher模塊控制所有組件和自動測量細(xì)胞；細(xì)胞形變由系統(tǒng)CCD相機自動記錄，并由CellEvaluator自動提取記錄顯微圖像形變數(shù)據(jù)，CellReporter可視化統(tǒng)計分析表征參數(shù)。在光學(xué)拉伸加載運行實驗中，科研學(xué)者可專注于闡述實驗結(jié)果
6)良好溫控微環(huán)境罩

單細(xì)胞牽張拉伸壓縮形變與機械力特性測試分析系統(tǒng)

中國代理服務(wù)商：北京思睿維科技有限公司馬金龍

單細(xì)胞牽張拉伸壓縮形變與機械力特性測試分析系統(tǒng)

Publications

RS ZELLTECHNIK BROCHURES

The Optical Stretcher

OPTICAL STRETCHER TECHNOLOGY

Lincoln, B., Schinkinger, S., Travis, K., Wottawah, F., Ebert, S., Sauer, F., Guck, J., 2007. Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications. Biomed. Microdevices 9, 703–710. doi:10.1007/s10544-007-9079-x
Ebert, S., Travis, K., Lincoln, B., Guck, J., 2007. Fluorescence ratio thermometry in a microfluidic dual-beam laser trap. Opt. Express 15, 15493–15499. doi:10.1364/OE.15.015493
Jensen-McMullin, C., Lee, H.P., Lyons, E.R.L., 2005. Demonstration of trapping, motion control, sensing and fluorescence detection of polystyrene beads in a multi-fiber optical trap. Opt. Express 13, 2634–2642. doi:10.1364/OPEX.13.002634
Wottawah, F., Schinkinger, S., Lincoln, B., Ananthakrishnan, R., Romeyke, M., Guck, J., K?s, J., 2005. Optical Rheology of Biological Cells. Phys. Rev. Lett. 94, 098103. doi:10.1103/PhysRevLett.94.098103
Lincoln, B., Erickson, H.M., Schinkinger, S., Wottawah, F., Mitchell, D., Ulvick, S., Bilby, C., Guck, J., 2004. Deformability-based flow cytometry.Cytometry A 59A, 203–209. doi:10.1002/cyto.a.20050

THEORETICAL MODELS

Ananthakrishnan, R., Guck, J., Wottawah, F., Schinkinger, S., Lincoln, B., Romeyke, M., Kas, J., 2005. Modelling the structural response of an eukaryotic cell in the optical stretcher. Curr. Sci. 88.
B. Bareil, P., Sheng, Y., Chiou, A., 2006. Local scattering stress distribution on surface of a spherical cell in optical stretcher. Opt. Express 14, 12503–12509. doi:10.1364/OE.14.012503
Bareil, P.B., Sheng, Y., Chen, Y.-Q., Chiou, A., 2007. Calculation of spherical red blood cell deformation in a dual-beam optical stretcher. Opt. Express 15, 16029–16034. doi:10.1364/OE.15.016029
Boyde, L., Ekpenyong, A., Whyte, G., Guck, J., 2012. Comparison of stresses on homogeneous spheroids in the optical stretcher computed with geometrical optics and generalized Lorenz–Mie theory. Appl. Opt. 51, 7934–7944. doi:10.1364/AO.51.007934
Ekpenyong, A.E., Posey, C.L., Chaput, J.L., Burkart, A.K., Marquardt, M.M., Smith, T.J., Nichols, M.G., 2009. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher. Appl. Opt. 48, 6344–6354. doi:10.1364/AO.48.006344
Teo, S.-K., Goryachev, A.B., Parker, K.H., Chiam, K.-H., 2010. Cellular deformation and intracellular stress propagation during optical stretching.Phys. Rev. E 81, 051924. doi:10.1103/PhysRevE.81.051924

CANCER RESEARCH AND DIAGNOSTICS

Kastl, L., Budde, B., Isbach, M., Rommel, C., Kemper, B., Schnekenburger, J., 2015. Optomechanical properties of cancer cells revealed by light-induced deformation and quantitative phase microscopy. pp. 952908–952908–6. doi:10.1117/12.2184764
Martin, M., Müller, K., Cadenas, C., Hermes, M., Zink, M., Hengstler, J.G., K?s, J.A., 2012. ERBB2 overexpression triggers transient high mechanoactivity of breast tumor cells. Cytoskeleton 69, 267–277. doi:10.1002/cm.21023
Fritsch, A., H?ckel, M., Kiessling, T., Nnetu, K.D., Wetzel, F., Zink, M., K?s, J.A., 2010. Are biomechanical changes necessary for tumour progression? Nat. Phys. 6, 730–732. doi:10.1038/nphys1800
Brunner, C., Niendorf, A., K?s, J.A., 2009. Passive and active single-cell biomechanics: a new perspective in cancer diagnosis. Soft Matter 5, 2171–2178. doi:10.1039/B807545J
Remmerbach, T.W., Wottawah, F., Dietrich, J., Lincoln, B., Wittekind, C., Guck, J., 2009. Oral Cancer Diagnosis by Mechanical Phenotyping. Cancer Res. 69, 1728–1732. doi:10.1158/0008-5472.CAN-08-4073
Martin, M., Mueller, K., Wottawah, F., Schinkinger, S., Lincoln, B., Romeyke, M., K?s, J.A., 2006. Feeling with light for cancer. p. 60800P–60800P–10. doi:10.1117/12.637899
Guck, J., Schinkinger, S., Lincoln, B., Wottawah, F., Ebert, S., Romeyke, M., Lenz, D., Erickson, H.M., Ananthakrishnan, R., Mitchell, D., K?s, J., Ulvick, S., Bilby, C., 2005. Optical Deformability as an Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence. Biophys. J. 88, 3689–3698. doi:10.1529/biophysj.104.045476

STEM CELL RESEARCH

Ekpenyong, A.E., Whyte, G., Chalut, K., Pagliara, S., Lautenschlaeger, F., Fiddler, C., Paschke, S., Keyser, U.F., Chilvers, E.R., Guck, J., 2012.Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent. Plos One 7, e45237. doi:10.1371/journal.pone.0045237
Galle, J., Bader, A., Hepp, P., Grill, W., Fuchs, B., Kas, J.A., Krinner, A., MarquaB, B., Muller, K., Schiller, J., Schulz, R.M., von Buttlar, M., von der Burg, E., Zscharnack, M., Loffler, M., 2010. Mesenchymal Stem Cells in Cartilage Repair: State of the Art and Methods to monitor Cell Growth, Differentiation and Cartilage Regeneration. Curr. Med. Chem. 17, 2274–2291. doi:10.2174/092986710791331095
Maloney, J.M., Nikova, D., Lautenschlager, F., Clarke, E., Langer, R., Guck, J., Van Vliet, K.J., 2010. Mesenchymal Stem Cell Mechanics from the Attached to the Suspended State. Biophys. J. 99, 2479–2487. doi:10.1016/j.bpj.2010.08.052
Lautenschl?ger, F., Paschke, S., Schinkinger, S., Bruel, A., Beil, M., Guck, J., 2009. The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc. Natl. Acad. Sci. 106, 15696–15701 doi:10.1073/pnas.0811261106

IMMUNE SYSTEM

Man, S.M., Ekpenyong, A., Tourlomousis, P., Achouri, S., Cammarota, E., Hughes, K., Rizzo, A., Ng, G., Wright, J.A., Cicuta, P., Guck, J.R., Bryant, C.E., 2014. Actin polymerization as a key innate immune effector mechanism to control Salmonella infection. Proc. Natl. Acad. Sci. 201419925 doi:10.1073/pnas.1419925111

BASIC RESEARCH

Schmidt, B.U.S., Kie?ling, T.R., Warmt, E., Fritsch, A.W., Stange, R., K?s, J.A., 2015. Complex thermorheology of living cells. New J. Phys. 17, 073010. doi:10.1088/1367-2630/17/7/073010
Chan, C.J., Ekpenyong, A.E., Golfier, S., Li, W., Chalut, K.J., Otto, O., Elgeti, J., Guck, J., Lautenschl?ger, F., 2015. Myosin II Activity Softens Cells in Suspension. Biophys. J. 108, 1856–1869. doi:10.1016/j.bpj.2015.03.009
Gladilin, E., Gonzalez, P., Eils, R., 2014. Dissecting the contribution of actin and vimentin intermediate filaments to mechanical phenotype of suspended cells using high-throughput deformability measurements and computational modeling. J. Biomech. 47, 2598–2605. doi:10.1016/j.jbiomech.2014.05.020
Maloney, J.M., Vliet, K.J.V., 2014. Chemoenvironmental modulators of fluidity in the suspended biological cell. Soft Matter. doi:10.1039/C4SM00743C
Warmt, E., Kie?ling, T.R., Stange, R., Fritsch, A.W., Zink, M., K?s, J.A., 2014. Thermal instability of cell nuclei. New J. Phys. 16, 073009. doi:10.1088/1367-2630/16/7/073009
Gyger, M., Stange, R., Kiessling, T.R., Fritsch, A., Kosnik, K.B., Beck-Sickinger, A.G., Zink, M., Kaes, J.A., 2014. Active contractions in single suspended epithelial cells. Eur. Biophys. J. Biophys. Lett. 43, 11–23. doi:10.1007/s00249-013-0935-8
Seltmann, K., Fritsch, A.W., K?s, J.A., Magin, T.M., 2013. Keratins significantly contribute to cell stiffness and impact invasive behavior. Proc. Natl. Acad. Sci. 201310493. doi:10.1073/pnas.1310493110
Maloney, J.M., Lehnhardt, E., Long, A.F., Van Vliet, K.J., 2013. Mechanical fluidity of fully suspended biological cells. Biophys. J. 105, 1767–1777. doi:10.1016/j.bpj.2013.08.040
Kie?ling, T.R., Stange, R., K?s, J.A., Fritsch, A.W., 2013. Thermorheology of living cells—impact of temperature variations on cell mechanics. New J. Phys. 15, 045026. doi:10.1088/1367-2630/15/4/045026
Kie?ling, T.R., Herrera, M., Nnetu, K.D., Balzer, E.M., Girvan, M., Fritsch, A.W., Martin, S.S., K?s, J.A., Losert, W., 2013. Analysis of multiple physical parameters for mechanical phenotyping of living cells. Eur. Biophys. J. 42, 383–394. doi:10.1007/s00249-013-0888-y
Paschke, S., Weidner, A.F., Paust, T., Marti, O., Beil, M., Ben-Chetrit, E., 2013. Technical advance: Inhibition of neutrophil chemotaxis by colchicine is modulated through viscoelastic properties of subcellular compartments. J. Leukoc. Biol. 94, 1091–1096. doi:10.1189/jlb.1012510
Chalut, K.J., H?pfler, M., Lautenschl?ger, F., Boyde, L., Chan, C.J., Ekpenyong, A., Martinez-Arias, A., Guck, J., 2012. Chromatin decondensation and nuclear softening accompany Nanog downregulation in embryonic stem cells. Biophys. J. 103, 2060–2070. doi:10.1016/j.bpj.2012.10.015
Matthews, H.K., Delabre, U., Rohn, J.L., Guck, J., Kunda, P., Baum, B., 2012. Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression. Dev. Cell 23, 371–383. doi:10.1016/j.devcel.2012.06.003
Mauritz, J.M.A., Esposito, A., Tiffert, T., Skepper, J.N., Warley, A., Yoon, Y.-Z., Cicuta, P., Lew, V.L., Guck, J.R., Kaminski, C.F., 2010. Biophotonic techniques for the study of malaria-infected red blood cells. Med. Biol. Eng. Comput. 48, 1055–1063. doi:10.1007/s11517-010-0668-0
Rusciano, G., 2010. Experimental analysis of Hb oxy–deoxy transition in single optically stretched red blood cells. Phys. Med. 26, 233–239. doi:10.1016/j.ejmp.2010.02.001

AGING PROCESSES

Schulze, C., Wetzel, F., Kueper, T., Malsen, A., Muhr, G., Jaspers, S., Blatt, T., Wittern, K.-P., Wenck, H., K?s, J.A., 2010. Stiffening of Human Skin Fibroblasts with Age. Biophys. J. 99, 2434–2442. doi:10.1016/j.bpj.2010.08.026

VESICLES

Delabre, U., Feld, K., Crespo, E., Whyte, G., Sykes, C., Seifert, U., Guck, J., 2015. Deformation of phospholipid vesicles in an optical stretcher. Soft Matter. doi:10.1039/C5SM00562K
Solmaz, M.E., Sankhagowit, S., Biswas, R., Mejia, C.A., Povinelli, M.L., Malmstadt, N., 2013. Optical stretching as a tool to investigate the mechanical properties of lipid bilayers. Rsc Adv. 3, 16632–16638. doi:10.1039/c3ra42510j
Solmaz, M.E., Biswas, R., Sankhagowit, S., Thompson, J.R., Mejia, C.A., Malmstadt, N., Povinelli, M.L., 2012. Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap. Biomed. Opt. Express 3, 2419–2427. doi:10.1364/BOE.3.002419

TECHNICAL ADVANCES

Grosser, S., Fritsch, A.W., Kie?ling, T.R., Stange, R., K?s, J.A., 2015. The lensing effect of trapped particles in a dual-beam optical trap. Opt. Express 23, 5221–5235. doi:10.1364/OE.23.005221
Bellini, N., Bragheri, F., Cristiani, I., Guck, J., Osellame, R., Whyte, G., 2012. Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher. Biomed. Opt. Express 3, 2658–2668. doi:10.1364/BOE.3.002658
Bellini, N., Vishnubhatla, K.C., Bragheri, F., Ferrara, L., Minzioni, P., Ramponi, R., Cristiani, I., Osellame, R., 2010. Femtosecond laser fabricated monolithic chip for optical trapping and stretching of single cells. Opt. Express 18, 4679–4688. doi:10.1364/OE.18.004679

中國代理服務(wù)商：北京思睿維科技有限公司馬金龍

關(guān)鍵詞：微流控芯片顯微鏡

熱門產(chǎn)品

產(chǎn)品名稱	參考價	地區(qū)	公司名稱	更新時間
雙層全溫恒溫恒濕振蕩培養(yǎng)箱生命科學(xué)儀器	￥19800	常州市	常州金壇精達(dá)儀器制造有限公司	2024-08-20	在線詢價
離心機轉(zhuǎn)子特征生命科學(xué)儀器	面議	常州市	常州金壇精達(dá)儀器制造有限公司	2024-04-08	在線詢價
微型手提式真空泵吸引器生命科學(xué)儀器	￥1300	上海市	上海精勝科學(xué)儀器有限公司	2023-11-24	在線詢價
光照培養(yǎng)箱 GXZ-600 生命科學(xué)儀器	￥5800	寧波市	寧波江南儀器廠	2022-12-07	在線詢價
臺式吸引器微型真空泵生命科學(xué)儀器	￥1430	上海市	上海精勝科學(xué)儀器有限公司	2023-11-24	在線詢價
微型臺式真空泵吸引器生命科學(xué)儀器	￥910	上海市	上海精勝科學(xué)儀器有限公司	2023-11-24	在線詢價

免責(zé)申明

所展示的信息由會員自行提供，內(nèi)容的真實性、準(zhǔn)確性和合法性由發(fā)布會員負(fù)責(zé)，儀表網(wǎng)對此不承擔(dān)任何責(zé)任。儀表網(wǎng)不涉及用戶間因交易而產(chǎn)生的法律關(guān)系及法律糾紛，糾紛由您自行協(xié)商解決

友情提醒：本網(wǎng)站僅作為用戶尋找交易對象，就貨物和服務(wù)的交易進行協(xié)商，以及獲取各類與貿(mào)易相關(guān)的服務(wù)信息的平臺。為避免產(chǎn)生購買風(fēng)險，建議您在購買相關(guān)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。過低的價格、夸張的描述、私人銀行賬戶等都有可能是虛假信息，請采購商謹(jǐn)慎對待，謹(jǐn)防欺詐，對于任何付款行為請您慎重抉擇！如您遇到欺詐等不誠信行為，請您立即與儀表網(wǎng)聯(lián)系，如查證屬實，儀表網(wǎng)會對該企業(yè)商鋪做注銷處理，但儀表網(wǎng)不對您因此造成的損失承擔(dān)責(zé)任！