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當(dāng)前位置:上海莼試生物技術(shù)有限公司>>分子生物學(xué)>>核酸純化與回收>> 海洋動(dòng)物組基因組DNA提取試劑盒操作步驟
PH調(diào)節(jié)粉末(PH9.18)保存運(yùn)輸
基因轉(zhuǎn)染試劑GenFectinTM(可做250T6孔板或35mm平皿轉(zhuǎn)
1 M Tris緩沖液 pH 8.0 滅菌保存運(yùn)輸
海洋動(dòng)物組基因組DNA提取試劑盒操作步驟儲(chǔ)存條件:避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
產(chǎn)品名稱:海洋動(dòng)物組基因組DNA提取試劑盒操作步驟
產(chǎn)品描述
本試劑盒采用高效、專一的吸附柱,特異結(jié)合核酸分子,可Z大限度去除雜質(zhì)蛋白及細(xì)胞中其他有機(jī)化合物。提取的基因組DNA片段大,純度高,質(zhì)量穩(wěn)定可靠。使用本試劑盒回收的DNA可適用于各種常規(guī)操作,包括酶切、PCR、文庫(kù)構(gòu)建、Southern雜交等實(shí)驗(yàn)。
產(chǎn)品優(yōu)勢(shì)
便捷:普通小型離心機(jī)即可完成。
快速:步驟少,操作簡(jiǎn)單,節(jié)省時(shí)間。
高效:20ug組織可以抽提到約40ug基因組DNA。
海洋動(dòng)物組基因組DNA提取試劑盒操作步驟儲(chǔ)存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴(yán)格按照產(chǎn)品包裝或者說(shuō)明書(shū)上的儲(chǔ)存條件進(jìn)行儲(chǔ)存,*可以保證其在有效期內(nèi)的正常使用。一般常見(jiàn)儲(chǔ)存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對(duì)于某些酶類制品,如果儲(chǔ)存條件是2-8攝氏度,不要儲(chǔ)存在零下,否則容易失活,對(duì)于某些需要嚴(yán)格避光,防潮,充氮?dú)獗Wo(hù)的,接觸空氣或者過(guò)分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品,運(yùn)輸途中冷藏問(wèn)題:
對(duì)于需要低溫儲(chǔ)存的產(chǎn)品,我們?cè)谶\(yùn)輸時(shí)候都會(huì)采用冷藏包裝,但是部分由于外界氣溫過(guò)高,運(yùn)輸時(shí)間較長(zhǎng),對(duì)于普通冷藏包裝,途中可能會(huì)溫度升高,這些都是沒(méi)有問(wèn)題的,因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲(chǔ)存條件,只是長(zhǎng)期儲(chǔ)存時(shí)候的條件,您可以放心使用。對(duì)于某些特殊的產(chǎn)品,如抗體,我們會(huì)采用干冰冷藏包裝,確保全程低溫。公司都對(duì)產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測(cè)試,行業(yè)經(jīng)驗(yàn)豐富。
海洋動(dòng)物組基因組DNA提取試劑盒操作步驟產(chǎn)品應(yīng)用:
對(duì)于一些相對(duì)特殊的產(chǎn)品,我們會(huì)提供產(chǎn)品說(shuō)明書(shū),里面會(huì)對(duì)產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實(shí)驗(yàn)方案進(jìn)行描述,您可以作為參考。對(duì)于常見(jiàn)試劑,因?yàn)閼?yīng)用廣泛,一般不提供說(shuō)明書(shū)。如果您在實(shí)驗(yàn)過(guò)程中遇到了問(wèn)題,歡迎與我們討論,我們將盡力幫您解決。
海洋動(dòng)物組基因組DNA提取試劑盒操作步驟分裝及溶液配制:
您收到貨物后不要自己進(jìn)行分包,因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請(qǐng)?jiān)谟嗁?gòu)時(shí)候與我們客服代表闡明,當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對(duì)于開(kāi)蓋后,長(zhǎng)期未使用的,請(qǐng)務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過(guò)長(zhǎng),超過(guò)產(chǎn)品有效期,建議您重新購(gòu)買,以免影響實(shí)驗(yàn)質(zhì)量。
CFN92838 http://www.chemfaces。。com/natural/Poricoic-acid-A-F-CFN92838.html C31H46O5 498.7 137551-38-3 Poricoic acid A(F) Triterpenoids >=98% 10mg
CFN92857 http://www.chemfaces。。com/natural/3-5-Dimethoxybenzylalcohol-CFN92857.html C9H12O3 168.2 705-76-0 3,5-Dimethoxybenzylalcohol Phenols >=95% 100mg
CFN92860 http://www.chemfaces。。com/natural/Bicucullinemethochloride-CFN92860.html C21H20NO6.Cl 417.8 53552-05-9 (-)-Bicuculline methochloride Alkaloids >=98% 20mg
CFN90597 http://www.chemfaces。。com/natural/Tracheloside-CFN90597.html C27H34O12 550.2 33464-71-0 Tracheloside Lignans >=98% 20mg
CFN97903 http://www.chemfaces。。com/natural/4-Dihydroxyacetophenone-CFN97903.html C8H8O3 152.2 1197-09-7 3',4'-Dihydroxyacetophenone Phenols >=98% 50mg
CFN97923 http://www.chemfaces。。com/natural/4-Hydroxyphenylacetic-acid-CFN97923.html C8H8O3 152.2 156-38-7 4-Hydroxyphenylacetic acid Phenols >=98% 100mg
CFN97949 http://www.chemfaces。。com/natural/Maltol-CFN97949.html C6H6O3 126.1 118-71-8 Maltol Flavonoids >=98% 100mg
CFN97963 http://www.chemfaces。。com/natural/Citropten-CFN97963.html C11H10O4 206.2 487-06-9 Citropten Coumarins >=98% 10mg
CFN97980 http://www.chemfaces。。com/natural/2-4-6-Trimethoxyacetophenone-CFN97980.html C11H14O4 210.2 832-58-6 2',4',6'-Trimethoxyacetophenone Phenols >=98% 20mg
CFN90561 http://www.chemfaces。。com/natural/Gossypol-CFN90561.html C30H30O8 518.56 303-45-7 Gossypol Phenols >=98% 20mg
CFN90565 http://www.chemfaces。。com/natural/Ginsenoside-Rg6-CFN90565.html C42H70O12 766.5 147419-93-0 Ginsenoside Rg6 Triterpenoids >=98% 10mg
CFN90566 http://www.chemfaces。。com/natural/7-Methoxycoumarin-CFN90566.html C10H8O3 176.2 531-59-9 7-Methoxycoumarin Coumarins > 98% 100mg not yet tested in other applications.
complex with RNPS1. Interacts with 蛋白分子量 predicted molecular weight: 43kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from mouse PGE2 C-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq].
Function : Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear.
Subunit : Homodimer. May interact with CEBPB.
Subcellular Location : Golgi apparatus membrane; Single-pass membrane protein. Nucleus. Note=According to PubMed:12050152, some fraction may be nuclear. Prostaglandin E synthase 2 truncated form: Cytoplasm. Note=Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form.
Tissue Specificity : Widely expressed. Expressed in brain, heart, liver, colon and lung.
Similarity : Belongs to the GST superfamily.
Contains 1 glutaredoxin domain.
Contains 1 GST C-terminal domain.
Database links : UniProtKB/Swiss-Prot: Q8BWM0.3
海洋動(dòng)物組基因組DNA提取試劑盒操作步驟英文名稱 Anti-duck hepatitis A virus polyprotein
中文名稱 鴨甲型肝炎聚蛋白抗體
別 名 polyprotein [duck hepatitis A virus]; DHAV; polyprotein (DHAV).
濃 度 1mg/1ml
規(guī) 格 1ml/1mg
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