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當(dāng)前位置:上海莼試生物技術(shù)有限公司>>分子生物學(xué)>>細(xì)胞培養(yǎng)及檢測(cè)>> RPMI 1640培養(yǎng)基粉末操作步驟
小鼠外周血單個(gè)核細(xì)胞分離液保存運(yùn)輸
產(chǎn)品名稱:RPMI 1640培養(yǎng)基粉末操作步驟
英文名字:RPMI 1640 Medium, Powder(Gibco)
物理性狀及指標(biāo):
谷氨酸:……………………L-谷氨酸
酚紅指示劑:………………含酚紅
形態(tài):………………………粉末
HEPES 緩沖溶液:………不含HEPES
丙酮酸鈉添加劑:…………不含丙酮酸鈉
培養(yǎng)基配方:
RPMI 1640, powder
RPMI 1640培養(yǎng)基粉末操作步驟描述:
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.
儲(chǔ)存條件:18-25℃保存,防潮密閉干燥。
RPMI 1640培養(yǎng)基粉末操作步驟產(chǎn)品應(yīng)用:
對(duì)于一些相對(duì)特殊的產(chǎn)品,我們會(huì)提供產(chǎn)品說明書,里面會(huì)對(duì)產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實(shí)驗(yàn)方案進(jìn)行描述,您可以作為參考。對(duì)于常見試劑,因?yàn)閼?yīng)用廣泛,一般不提供說明書。如果您在實(shí)驗(yàn)過程中遇到了問題,歡迎與我們討論,我們將盡力幫您解決。
務(wù)分裝及溶液配制:
您收到貨物后不要自己進(jìn)行分包,因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請(qǐng)?jiān)谟嗁彆r(shí)候與我們客服代表闡明,當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對(duì)于開蓋后,長期未使用的,請(qǐng)務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過長,超過產(chǎn)品有效期,建議您重新購買,以免影響實(shí)驗(yàn)質(zhì)量。
RPMI 1640培養(yǎng)基粉末操作步驟儲(chǔ)存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴(yán)格按照產(chǎn)品包裝或者說明書上的儲(chǔ)存條件進(jìn)行儲(chǔ)存,*可以保證其在有效期內(nèi)的正常使用。一般常見儲(chǔ)存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對(duì)于某些酶類制品,如果儲(chǔ)存條件是2-8攝氏度,不要儲(chǔ)存在零下,否則容易失活,對(duì)于某些需要嚴(yán)格避光,防潮,充氮?dú)獗Wo(hù)的,接觸空氣或者過分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品,運(yùn)輸途中冷藏問題:
對(duì)于需要低溫儲(chǔ)存的產(chǎn)品,我們?cè)谶\(yùn)輸時(shí)候都會(huì)采用冷藏包裝,但是部分由于外界氣溫過高,運(yùn)輸時(shí)間較長,對(duì)于普通冷藏包裝,途中可能會(huì)溫度升高,這些都是沒有問題的,因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲(chǔ)存條件,只是長期儲(chǔ)存時(shí)候的條件,您可以放心使用。對(duì)于某些特殊的產(chǎn)品,如抗體,我們會(huì)采用干冰冷藏包裝,確保全程低溫。公司都對(duì)產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測(cè)試,行業(yè)經(jīng)驗(yàn)豐富。
RPMI 1640培養(yǎng)基粉末操作步驟儲(chǔ)存條件:避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
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CFN97196 http://www.chemfaces。。com/natural/2-Hydroxy-5-methoxyacetophenone-CFN97196.html C9H10O3 166.2 705-15-7 2'-Hydroxy-5'-methoxyacetophenone Phenols > 95% 100mg
CFN97218 http://www.chemfaces。。com/natural/p-Coumaric-acid-ethyl-ester-CFN97218.html C11H12O3 192.2 7362-39-2 p-Coumaric acid ethyl ester Phenylpropanoids > 98% 100mg
CFN97225 http://www.chemfaces。。com/natural/8-Geranyloxypsoralen-CFN97225.html C21H22O4 338.4 7437-55-0 8-Geranyloxypsoralen Coumarins > 95% 20mg
CFN97233 http://www.chemfaces。。com/natural/Obacunone-CFN97233.html C26H30O7 454.5 751-03-1 Obacunone Triterpenoids >=98% 20mg
CFN97235 http://www.chemfaces。。com/natural/alpha-Tocopherolquinone-CFN97235.html C29H50O3 446.7 7559-04-8 alpha-Tocopherolquinone Quinones > 95% 20mg
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CFN97252 http://www.chemfaces。。com/natural/Cleomiscosin-A-CFN97252.html C20H18O8 386.4 76948-72-6 Cleomiscosin A Coumarins 98% 5mg
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response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by RPMI 1640培養(yǎng)基粉末操作步驟phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The
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