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小鼠外周血單個(gè)核細(xì)胞分離液保存運(yùn)輸
產(chǎn)品名稱:2×HEPES(pH7.2-7.4)售后服務(wù)
英文名字:2×HEPES(pH7.2-7.4)
產(chǎn)品描述
HEPES 即羥乙基呱嗪乙硫磺酸(N’-a-hydroxythylpiperazine-N’-ethanesulfanic acid )是一種氫離子緩沖劑,能較長時(shí)間控制恒定的pH 范圍。有效緩沖范圍為pH6.8-8.2。HEPES 用于細(xì)胞培養(yǎng)時(shí)使用終濃度為10-25mM,在開放式培養(yǎng)或細(xì)胞觀察時(shí)能維持較恒定的PH 值。4℃保存可延長產(chǎn)品保質(zhì)期,長期不用建議低溫保存。
產(chǎn)品優(yōu)勢
常用于配制蛋白提取緩沖液、細(xì)胞培養(yǎng)用緩沖液等。
儲(chǔ)存條件
室溫保存。
注意事項(xiàng)?
為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
2×HEPES(pH7.2-7.4)售后服務(wù)儲(chǔ)存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴(yán)格按照產(chǎn)品包裝或者說明書上的儲(chǔ)存條件進(jìn)行儲(chǔ)存,*可以保證其在有效期內(nèi)的正常使用。一般常見儲(chǔ)存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對于某些酶類制品,如果儲(chǔ)存條件是2-8攝氏度,不要儲(chǔ)存在零下,否則容易失活,對于某些需要嚴(yán)格避光,防潮,充氮?dú)獗Wo(hù)的,接觸空氣或者過分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品,運(yùn)輸途中冷藏問題:
對于需要低溫儲(chǔ)存的產(chǎn)品,我們在運(yùn)輸時(shí)候都會(huì)采用冷藏包裝,但是部分由于外界氣溫過高,運(yùn)輸時(shí)間較長,對于普通冷藏包裝,途中可能會(huì)溫度升高,這些都是沒有問題的,因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲(chǔ)存條件,只是長期儲(chǔ)存時(shí)候的條件,您可以放心使用。對于某些特殊的產(chǎn)品,如抗體,我們會(huì)采用干冰冷藏包裝,確保全程低溫。公司都對產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測試,行業(yè)經(jīng)驗(yàn)豐富。
2×HEPES(pH7.2-7.4)售后服務(wù)分裝及溶液配制:
您收到貨物后不要自己進(jìn)行分包,因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請?jiān)谟嗁彆r(shí)候與我們客服代表闡明,當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對于開蓋后,長期未使用的,請務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過長,超過產(chǎn)品有效期,建議您重新購買,以免影響實(shí)驗(yàn)質(zhì)量。
2×HEPES(pH7.2-7.4)售后服務(wù)產(chǎn)品應(yīng)用:
對于一些相對特殊的產(chǎn)品,我們會(huì)提供產(chǎn)品說明書,里面會(huì)對產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實(shí)驗(yàn)方案進(jìn)行描述,您可以作為參考。對于常見試劑,因?yàn)閼?yīng)用廣泛,一般不提供說明書。如果您在實(shí)驗(yàn)過程中遇到了問題,歡迎與我們討論,我們將盡力幫您解決。
2×HEPES(pH7.2-7.4)售后服務(wù)儲(chǔ)存條件:避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
CFN99518 http://www.chemfaces。。com/natural/Muscone-CFN99518.html C16H30O 238.42 541-91-3 Muscone Miscellaneous >=98% 0.15ml
CFN99519 http://www.chemfaces。。com/natural/Dryocrassin-ABBA-CFN99519.html C43H48O16 820.81 12777-70-7 Dryocrassin ABBA Phenols >=98% 10mg
CFN99478 http://www.chemfaces。。com/natural/Cinnamaldehyde-CFN99478.html C9H8O 132.2 104-55-2 Cinnamaldehyde Phenylpropanoids >=98% 20mg
CFN99522 http://www.chemfaces。。com/natural/Plantamajoside-CFN99522.html C29H36O16 640.6 104777-68-6 Plantamajoside Phenylpropanoids >=98% 20mg
CFN99523 http://www.chemfaces。。com/natural/Monotropein-CFN99523.html C16H22O11 390.34 5945-50-6 Monotropein Iridoids >=98% 10mg
CFN99524 http://www.chemfaces。。com/natural/Crotonoside-CFN99524.html C10H13N5O5 283.24 1818-71-9 Crotonoside Alkaloids >=98% 20mg
CFN99525 http://www.chemfaces。。com/natural/Complanatoside-A-CFN99525.html C27H30O18 642.5 146501-37-3 Complanatoside A Flavonoids >=98% 20mg
CFN99532 http://www.chemfaces。。com/natural/Anemarsaponin-B-CFN99532.html C45H74O18 903.04 139051-27-7 Anemarsaponin B Steroids >=98% 20mg
CFN99533 http://www.chemfaces。。com/natural/Anemarsaponin-E-CFN99533.html C46H78O19 934.03 136565-73-6 Anemarsaponin E Steroids >=98% 10mg
Function : Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Subunit : Interacts with AXIN1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Binds DNA as a homotetramer. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1, and recruits them to promoters. In vitro, the interaction of TP53 with cancer-associated/HPV (E6) viral proteins leads to ubiquitination and degradation of TP53 giving a possible model for cell growth regulation. This complex formation requires 2×HEPES(pH7.2-7.4)售后服務(wù)an additional factor, E6-AP, which stably associates with TP53 in the presence of E6. Interacts (via C-terminus) with TAF1; when TAF1 is part of the TFIID complex. Interacts with ING4; this interaction may be indirect. Found in a complex with CABLES1 and TP73. Interacts with HIPK1, HIPK2, and TP53INP1. Interacts with WWOX.
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