Reagent: Polyplus DNA高效轉(zhuǎn)染試劑.

Molecule delivered: Plasmid DNA.

Applications:Transient and stable gene expression from plasmid DNA transfection CRISPR Genome editing using DNA approach.

Cell types :Adherent hard-to-transfect mammalian cell:

- Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…)

- Stem cells (embryonic, mesenchymal, induced pluripotent)

- Cancer cell lines

Number of transfections : 1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol.

Storage: Store jetOPTIMUS® at 5℃±3℃.

Provided with: jetOPTIMUS® Buffer.

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.