Sigma RPMI-1640 Medium(without L-glutamine)

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: yes;L-glutamine: yes;sodium pyruvate: 0.055 g/L;phenol red: yes;

glucose: 3.15 g/L.

General description:Sigma RPMI-1640 Medium(without L-glutamine), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Application:Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:

has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells

has been used to culture the cartilage biopsies

may be used for the propagation of Cowdria ruminantium