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Takara

培養(yǎng)的細胞生長緩慢的原因及解決方法都有哪些?

時間:2023/10/16閱讀:343
分享:

我們在培養(yǎng)細胞的過程中可能會遇到各式各樣的問題,可見細胞的嬌氣程度,本文針對培養(yǎng)的細胞生長緩慢的原因及解決方法都有哪些進行探討,一起來學習一下吧!

可能原因

1.由于更換不同培養(yǎng)液或血清;

2.培養(yǎng)液中一些細胞生長必須成分如谷an酰胺或生長因子耗盡或缺乏或已被破壞;

3.培養(yǎng)物中有少量細菌或真菌污染;

4.試劑保存不當;

5.接種細胞起始濃度太低;

6.細胞已老化;

7.支原體污染。

解決方法

1.比較新培養(yǎng)液與原培養(yǎng)液成分,比較新血清與舊血清支持細胞生長實驗,讓細胞逐漸適應新培養(yǎng)液;

2.換入新鮮配置培養(yǎng)液,或補加谷an酰胺及生長因子;

3.用無抗生素培養(yǎng)液培養(yǎng),如發(fā)現(xiàn)污染,丟棄培養(yǎng)物;

4.血清需保存在-10到-20℃。培養(yǎng)液需在2-8℃避光保存。含血清完quan培養(yǎng)液在2-8℃保存,需在1周內用完;

5.增加接種細胞起始濃度;

6.換用新的保種細胞;

7.分離培養(yǎng)物,檢測支原體。清潔支架和培養(yǎng)箱。如發(fā)現(xiàn)支原體污染,丟棄培養(yǎng)物。

以上就是關于培養(yǎng)的細胞生長緩慢的原因及解決方法都有哪些的問題解答,如果你想了解更多請咨詢百奧創(chuàng)新客服哦~


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