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轉(zhuǎn)染試劑合集

時(shí)間:2023/5/15閱讀:623
分享:

線性PEI轉(zhuǎn)染試劑簡(jiǎn)介:
線性 PEI轉(zhuǎn)染試劑是一種高電荷陽離子聚合物,非常容易帶負(fù)電荷的核酸分子結(jié)合,形成復(fù)合物, 并使該復(fù)合物進(jìn)入細(xì)胞中。PEI轉(zhuǎn)染試劑是一種瞬時(shí)轉(zhuǎn)染試劑,細(xì)胞毒性低,轉(zhuǎn)染效率高,在H293  CHO 等細(xì)胞中基因表達(dá)效率較高。目前已經(jīng)驗(yàn)證線性 PEI 轉(zhuǎn)染試劑廣泛適用于多種細(xì)胞系。例如:H293CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2  Hela 細(xì)胞等。該試劑與含血清的培養(yǎng)基兼容,能高效的將核酸導(dǎo)入細(xì)胞。


北京百奧創(chuàng)新科技有限公司代理以下品牌的線性PEI轉(zhuǎn)染試劑


品牌產(chǎn)品貨號(hào)產(chǎn)品名稱規(guī)格
SerochemPRIME-P100-100MGPRIME-P100-1GPEI Prime? 粉末,轉(zhuǎn)染級(jí)線性聚乙烯亞胺100mg/1g
Prime-AQ100-5ML
Prime-AQ100-100ML
PEI Prime? AQ 1 mg/mL 液體轉(zhuǎn)染試劑5mL/100mL
Polysciences24765-1
24765-100
PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)100mg/1g
23966-1
23966-100
Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K)100mg/1g
Polyplus114-15jetPRIME Transfection Reagent0.1 mL


產(chǎn)品展示:

       


脂質(zhì)體轉(zhuǎn)染試劑簡(jiǎn)介:
脂質(zhì)體是磷脂分散在水中時(shí)形成的脂質(zhì)雙分子層,具有膜的融合及內(nèi)吞作用,可用作外源物質(zhì)進(jìn)入細(xì)胞的載體。脂質(zhì)體轉(zhuǎn)染試劑原理:陽離子脂質(zhì)體表層帶正電荷,能與核酸的磷酸根通過靜電作用將 DNA分子包裹內(nèi),形成DNA脂復(fù)合體,也能被表面帶負(fù)電荷的細(xì)胞膜吸附,再通過膜的融合或細(xì)胞的內(nèi)吞作用,將DNA傳遞進(jìn)入細(xì)胞。由于這種轉(zhuǎn)染方法具有好的重現(xiàn)性和較高的轉(zhuǎn)染效率,不僅適用于貼壁細(xì)胞,對(duì)懸浮細(xì)胞也適用。

北京百奧創(chuàng)新科技有限公司代理以下品牌的脂質(zhì)體轉(zhuǎn)染試劑


品牌產(chǎn)品貨號(hào)產(chǎn)品名稱規(guī)格
Polyplus101000122in vivo-jetRNA®+ transfection reagent1 mL
ThermoFisher11668019Lipofectamine2000 transfection reagent1.5 mL
L3000015Lipofectamine3000 transfection reagent1.5 mL


產(chǎn)品展示:

 


RNAi轉(zhuǎn)染試劑簡(jiǎn)介:
陽離子脂質(zhì)體轉(zhuǎn)染試劑的選擇對(duì)于利用siRNA介導(dǎo)的基因沉默實(shí)驗(yàn)來說同樣是至關(guān)重要的。沒有高效的轉(zhuǎn)染,就不能有效的引發(fā)相應(yīng)的細(xì)胞響應(yīng),會(huì)直接影響同一siRNA的抑制效果。只有將siRNA高效轉(zhuǎn)染哺乳動(dòng)物細(xì)胞,才能更真實(shí)的檢測(cè)siRNA干擾基因表達(dá)的效果。如何選擇適合的轉(zhuǎn)染試劑和轉(zhuǎn)染條件,往往取決于不同的哺乳動(dòng)物細(xì)胞類型和不同的核酸分子。

北京百奧創(chuàng)新科技有限公司代理以下品牌的RNAi轉(zhuǎn)染試劑


品牌產(chǎn)品貨號(hào)產(chǎn)品名稱規(guī)格
多納DN001- 01CALNPRNAi in vitroB0.1 mL;A0.5 mL
DN001- 05B0.5 mL;A1 mL x 2
DN001- 10B1.0 mL;A1 mL x 4


產(chǎn)品展示:


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