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昆蟲細胞培養(yǎng)基的發(fā)展

時間:2022/10/20閱讀:260
分享:

昆蟲細胞培養(yǎng)基為昆蟲細胞的生長、增殖、分化提供了重要保障。昆蟲細胞培養(yǎng)基的發(fā)展經歷了天然培養(yǎng)基、合成培養(yǎng)基和無血清培養(yǎng)基三個階段。隨著昆蟲桿狀病毒表達載體系統(tǒng)(BEVS)的廣泛應用,以草地貪夜蛾細胞株Sf9Sf21,以及粉紋夜蛾細胞株Tn5B1-4(商品名High Five)為主的昆蟲細胞體外培養(yǎng)越來越受重視,昆蟲細胞培養(yǎng)基也越來越受到關注。目前應用廣泛的商品化基礎培養(yǎng)基主要是Grace’s培養(yǎng)基、TC-100培養(yǎng)基和IPL-41培養(yǎng)基。

微信截圖_20221019132030.png

 現(xiàn)有的培養(yǎng)基是在分析昆蟲血淋巴的化學組成基礎上發(fā)展和形成的。后來研究發(fā)現(xiàn)在合成培養(yǎng)基中加入動物血清(小牛血清或胎牛血清),能更有效地促進細胞的生長與繁殖。以上三種商業(yè)化培養(yǎng)基在應用中一般是補加3%-15%的血清用于昆蟲細胞培養(yǎng)。但以血清作為添加物,存在許多問題,如血清的來源、質量、價格、批間差異和重復性的問題;血清還是支原體和其他異源病毒的重要來源;另外,血清成分復雜,給基因工程表達產物的后處理帶來困難,因此昆蟲細胞無血清培養(yǎng)基的開發(fā)就顯得尤為重要。近年來,隨著無血清細胞培養(yǎng)技術的迅速發(fā)展,已有多種細胞株系在相應的無血清培養(yǎng)基中生長和增殖獲得成功。如北京百奧創(chuàng)新科技有限公司提供的昆蟲細胞無血清培養(yǎng)基滿足Sf9、Sf21High Five和果蠅S2等細胞的生長,具體的適用性請咨詢百奧創(chuàng)新的客服。

品牌

產品名稱

貨號

規(guī)格

Expressions Systems

ESF 921 Insect Cell Culture Medium, Protein Free

96-001-01

1 L Bottle

昆蟲細胞培養(yǎng)基.jpg

1.png




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