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血清必須要熱滅活嗎?

時(shí)間:2022/10/12閱讀:271
分享:

血清是生長和附著因子、礦物質(zhì)、脂質(zhì)和激素的來源,是細(xì)胞培養(yǎng)的重要組分。血清能調(diào)節(jié)細(xì)胞膜的通透性,同時(shí)能作為載體攜帶酶、脂質(zhì)、酶和微量元素等進(jìn)入細(xì)胞。在使用的過程中,到底是選擇沒有滅活的血清?還是選擇熱滅活血清?有啥區(qū)別嗎?

熱滅活可以破壞血清中補(bǔ)體的活性。哺乳動(dòng)物血清中存在的補(bǔ)體蛋白參與細(xì)胞的溶解,收縮平滑肌,刺激細(xì)胞和血小板釋放組胺,并激活淋巴細(xì)胞和巨噬細(xì)胞。有研究比較了正常熱滅活胎牛血清對(duì)各種細(xì)胞株的生長能力影響(如下圖所示),發(fā)現(xiàn)11株細(xì)胞中,熱滅活對(duì)6株細(xì)胞有負(fù)面影響(紅框)、3株無影響(綠框)、2株有微弱正面改善(黃框),因此在多數(shù)細(xì)胞培養(yǎng)中血清不需要熱滅活,熱滅活對(duì)細(xì)胞生長沒有明顯的促進(jìn)作用,反而還會(huì)影響血清質(zhì)量,使細(xì)胞的生長速率降低。因此如果不是非必須,可不必做血清熱滅活這一處理。所以一般的細(xì)胞選擇沒有滅活的血清即可。

3352434614110409915-14.jpg

圖1 使用未滅活或滅活血清的細(xì)胞生長狀況

但是有些細(xì)胞需要選擇熱滅活血清,如胚胎干細(xì)胞(ESC)、平滑肌細(xì)胞、免疫細(xì)胞和昆蟲細(xì)胞培養(yǎng)。

北京百奧創(chuàng)新科技有限公司提供熱滅活/非熱滅活各式胎牛和新生牛血清以供實(shí)驗(yàn)的選擇,產(chǎn)品詳情,請(qǐng)咨詢客服小姐姐。

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