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有趣又苦逼的實(shí)驗(yàn)室日常(二)——細(xì)胞轉(zhuǎn)染專題

時(shí)間：2022/5/12閱讀：478
分享：

有趣又苦逼的實(shí)驗(yàn)室日常(二)——細(xì)胞轉(zhuǎn)染專題

1. 質(zhì)粒的小船說(shuō)翻就翻，轉(zhuǎn)染的巨輪說(shuō)沉就沉，唯有實(shí)驗(yàn)"狗"的信心屹立不倒。

圖片1.png

 

2. 轉(zhuǎn)染十年功，準(zhǔn)備不足三分鐘；轉(zhuǎn)染十年功，細(xì)胞污染三分鐘；轉(zhuǎn)染十年功，質(zhì)粒不純三分鐘；轉(zhuǎn)染十年功，細(xì)胞不好三分鐘；轉(zhuǎn)染十年功，試劑不對(duì)三分鐘！

圖片2.png

 

3. 好做就是好做，難做就是難做，你們說(shuō)細(xì)胞轉(zhuǎn)染好難做是什么意思?

圖片3.png

 

4. 做實(shí)驗(yàn)的人換了一批又一批，只有我，雷打不動(dòng)地做細(xì)胞轉(zhuǎn)染。

5. 優(yōu)質(zhì)質(zhì)粒是美術(shù)，轉(zhuǎn)染效率高是技術(shù)，熒光蛋白表達(dá)是藝術(shù)，流式失敗是手術(shù)。

圖片4.png

 

6. 至今轉(zhuǎn)染不成功的原因：質(zhì)粒不好下手，試劑不好開口。

圖片5.png

 

7. 做人要是能像熒光蛋白表達(dá)一樣明顯就好了。

圖片6.png

 


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