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甲基化 DNA 檢測(cè)：MSP-ZEWB 提取亞硫酸氫鈉處理 DNA

時(shí)間：2020/6/15閱讀：1615
分享：

檢測(cè)DNA 甲基化需多個(gè)技術(shù)環(huán)節(jié)：制備基因組 DNA 樣品；DNA的片段化，再用亞硫酸氫鈉處理，使未甲基化 C 轉(zhuǎn)變成 U 而甲基化 C 保持不變； 純化亞硫酸氫鈉處理后的痕量DNA；用qPCR 或二代測(cè)序等檢測(cè)。

MSP-ZEWB 提取基因組DNA 效力與常用硅醇磁珠相當(dāng)。 MSP-ZEWB 純化亞硫酸氫鈉處理后痕量 DNA 的片段耐受高濃度陰離 子干擾 (參見(jiàn)：IntJBiolMacromol2018;120(B):2234-2241)。

MSP-ZEWB 提取短鏈核酸效力強(qiáng)，屬于其優(yōu)勢(shì)應(yīng)用之一。

磁珠特點(diǎn)
? 核-殼結(jié)構(gòu)：聚合物包裹納米磁芯
? 超順磁納米 Fe3O4顆粒質(zhì)量占比高
? 磁響應(yīng)速度不遜于常用競(jìng)品
? 表面有小體積兼性離子對(duì)修飾層
? 蛋白及中性小分子非特異性吸附低
? 表層吸附大量水，分散穩(wěn)定性好
? 分離性能標(biāo)定值批間波動(dòng)<6%

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