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提取痕量樣品中核酸：兩性解離離子交換磁珠 MSP-ZEWB

時(shí)間：2020/5/25閱讀：376
分享：

兩性解離離子交換磁珠 MSP-ZEWB 為明星產(chǎn)品，表面有兼性離子對(duì) 修飾層，脂肪族羧基、脂肪族氨基及咪唑基組成的兩性解離基團(tuán)；pH~4.2 經(jīng)靜電吸引結(jié)合陰離子(核酸)，pH~8.6 時(shí)靜電排斥洗脫陰離子(核酸)。
兩性解離離子交換磁珠結(jié)合樣品中帶同種電荷離子時(shí)基本無(wú)選擇性，
但磁珠與核酸之間的靜電吸引顯著強(qiáng)于硅醇磁珠與核酸之間的氫鍵。
提取痕量樣品(如脫落細(xì)胞)中核酸是 MSP-ZEWB 的優(yōu)勢(shì)應(yīng)用之一。 提取RNA 備選流程：終濃度>3.0M 鹽酸胍變性樣品中蛋白；吸附緩 沖液稀釋并調(diào)節(jié)混合物pH 到 3.4~4.2，加磁珠吸附 RNA；洗滌、洗脫RNA； 如需去除所有 DNA，則用無(wú) RNA 酶的 DNA 酶 I 處理所得 RNA，再提取。 提取DNA 時(shí)用蛋白酶 K 替換鹽酸胍，用于滅活 DNA 酶。
 

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