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Norgen植物病原體檢測試劑盒

時間:2020/2/28閱讀:492
分享:

植物病原體包括真菌,細(xì)菌,線蟲和病毒,所有可導(dǎo)致疾病癥狀并顯著降低生產(chǎn)力,質(zhì)量甚至導(dǎo)致植物死亡的生物。病原菌可以多種方式引入并傳播到宿主植物中。細(xì)菌和真菌孢子可以通過風(fēng),雨傳遞,也可以通過雨水從土壤中轉(zhuǎn)移到植物組織中。當(dāng)昆蟲以被感染的宿主植物為食,再以未感染的植物為食并進食時,它們可以作為病原體感染植物的媒介。病原體還可以通過受感染的種子,移植或受污染的設(shè)備,灌溉用水和人類等方式傳播。

 

 

       為了助力廣大科研工作者對植物病原體的研究,北京百奧創(chuàng)新作為專業(yè)的生命科學(xué)領(lǐng)域解決方案供應(yīng)商,為您推薦Norgen品牌的植物病原體(細(xì)菌,真菌,病毒和類病毒)檢測試劑盒,專門用于檢測植物病原體,僅供研究使用,不得用于體外診斷。

 

指標(biāo)英文名稱病原體指標(biāo)類型TaqMan PCR終點PCR
Erwinia amylovora解淀粉歐文氏菌細(xì)菌TM35100EP35100
Aspergillus niger黑曲霉真菌TM32900EP32900
Botrytis cinerea葡萄孢菌真菌TM29400EP29400
Cladosporium cladosporioides枝孢梭菌真菌TM33000EP33000
Penicillium sp.青霉菌真菌TM33200EP33200
Phomopsis viticola葡萄生擬莖點菌真菌TM34900EP34900
Avocado Sunblotch Viroid (ASBVd)鱷梨日斑類病毒類病毒TM38800EP38800
Chrysanthemum Chlorotic Mottle Viroid (CChMVd)菊黃斑狀病毒類病毒TM35500EP35500
Chrysanthemum Stunt Viroid (CSVd)菊矮化類病毒類病毒TM39900EP39900
Citrus Exocortis Viroid (CEVd)柑桔裂皮類病毒類病毒TM47600EP47600
Coconut Cadang-Cadang Viroid (CCCVd)椰子死亡類病毒類病毒TM47900EP47900
Coconut Tinangaja Viroid (CTiVd)椰子敗生類病毒類病毒TM50500EP50500
Hop Stunt Viroid (HSVd)啤酒花矮化類病毒類病毒TM38900EP38900
Hop Latent Viroid (HLVd)啤酒花潛隱類病毒類病毒TM38700EP38700
Pospiviroid馬鈴薯紡錘塊莖類病毒類病毒TM53700EP53700
Potato Spindle Tuber Viroid (PSTVd)馬鈴薯紡錘塊莖類病毒類病毒TM38600EP38600
Tomato Chlorotic Dwarf Viroid (TCDVd)番茄褪綠矮小病毒類病毒TM39000EP39000
Tomato Planta Macho Viroid (TPMVd)番茄雄株類病毒類病毒TM48000EP48000
Plum Pox Virus (PPV)李痘病毒病毒TM33400EP33400
Saccharomyces cerevisiae釀酒酵母酵母TM33300EP33300
Grapevine Red Blotch Virus (GRBV)葡萄紅斑點病毒病毒TM65800——
Candidatus Liberibacter黃龍病菌細(xì)菌TM64300EP64300
Candidatus Liberibacter solanacearum茄科植物韌皮部桿菌細(xì)菌TM66200——
Xylella fastidiosa木質(zhì)部難養(yǎng)菌細(xì)菌TM67000EP67000

 

       PS: 所有試劑盒均已開發(fā)并驗證可與以下PCR儀器一起使用:

       Qiagen Rotor-Gene Q

       BioRad CFX96 TouchTM 實時PCR檢測系統(tǒng)

 

       每種病原體有兩種形式檢測試劑盒,分別是:

 

 

 

        TaqMan PCR檢測試劑盒特點:

 

        1.PCR對照以監(jiān)測PCR抑制情況并驗證質(zhì)量;

        2.用于目標(biāo)和PCR對照檢測的預(yù)混液;

        3.引物和探針混合物;

        4.陽性對照和陰性對照以確認(rèn)試劑盒試劑的完整性。

 

 

       TaqMan PCR陽性結(jié)果示例。高于基線的FAM和HEX通道的兩個PCR信號均表示PCR成功。

結(jié)果歷史交鋒
     

 

       終點PCR檢測試劑盒特點:

 

       1.用于病原體基因組區(qū)域特異性擴增的預(yù)混液和引物;

       2.陽性對照和陰性對照以確認(rèn)試劑盒試劑的完整性;

       3.加載染料和DNAladder以方便結(jié)果分析。

 

 

       不同濃度下的Pospiviroid(馬鈴薯紡錘塊莖類病毒)的擴增在1X TAE,1.4%瓊脂糖凝膠中的展示。Pospiviroid靶標(biāo)擴增的大小對應(yīng)于由所提供的DNA Marker(M)代表的184 bp條帶。

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