公司提供羅丹明標記的羊抗人IgM操作步驟質(zhì)量*，產(chǎn)品齊全，保證實驗的有效性，咨詢。 免費送貨上門。
英文名稱Goat Anti-human IgM/RBITC

中文名稱：羅丹明標記的羊抗人IgM操作步驟

規(guī)格：0.3ml

說 明 書0.3ml  

抗體來源Goat

克隆類型Polyclonal

交叉反應 hu

產(chǎn)品應用IF=1:100-1000  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量900kDa

性    狀Lyophilized or Liquid

濃    度2mg/1ml

免 疫 原Full length plasma protein

亞    型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C.

產(chǎn)品介紹background:

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

抗體的鑒定

篩選雜交瘤細胞通過選擇性培養(yǎng)而獲得雜交細胞系中，僅少數(shù)能分泌針對免疫原的特異性抗體。一般在雜交瘤細胞布滿孔底1／10面積時，即可開始檢測特異性抗體，篩選出所需要的雜交瘤細胞系?！?/span>
檢測抗體的方法應根據(jù)抗原的性質(zhì)、抗體的類型不同，選擇不同的篩選方法，一般以快速、簡便、特異、敏感的方法為原則。
常用的方法有:
　　1.　ELISA用于可溶性抗原(蛋白質(zhì))、細胞和病毒等McAb的檢測。
　　2.　RIA用于可溶性抗原、細胞McAb的檢測。
　　3.　FACS(熒光激活細胞分類儀)用于檢查細胞表面抗原的McAb檢測。
　　4.　IFA用于細胞和病毒McAb的檢測。
可靠的篩選方法必須在融合前建立，避免由于方法不當貽誤整個篩選時機。
應用范圍：
   1.免疫組織及免疫病理中抗原、抗體檢查，如：各腫瘤抗原檢查，細胞凋亡檢測。
   2.用于細胞因子等檢查的熒光流式細胞儀測定。
   3.用于臨床自身免疫病的多種自身抗體熒光檢查，如：抗核抗體，抗心肌酶抗體。
   4.白細胞分型抗原的免疫熒光染色。
   5.用于細菌、病毒、梅毒螺旋體、鉤端螺旋體等微生物的抗原及抗體檢測。
主要性能：
0.5ml或根據(jù)試驗需求的各種包裝，并提供濃縮液或凍干粉，含有0.01%NaN3防腐劑。 β-arrestin 1蛋白IgG含量在2mg/ml。FITC為Sigma異構體I，約在70-80ug/ml，495nm為zui大吸收峰，激發(fā)波長 525 nm 。
Rabbit Anti-chicken IgM/FITC        FITC標記的兔抗雞IgM     0.3ml 人蛋白    Human Nephrin ELISA Kit    人蛋白     96T
Rabbit Anti-chicken IgM/HRP        辣根過氧化物酶標記的兔抗雞IgM     0.1ml 小鼠補體蛋白3    C3(Mouse Complement 3) ELISA Kit    小鼠補體蛋白3     96T
Rabbit Anti-chicken IgM/Cy3        Cy3標記的兔抗雞IgM     0.1ml Kelch樣蛋白3抗體    KLHL3    Kelch樣蛋白3抗體     0.2ml
Rabbit Anti-chicken IgM/RBITC        羅丹明標記的兔抗雞IgM     0.3ml 單純皰疹Ⅰ型病毒 IgM（捕獲法二步法）    HSV- I IgM    單純皰疹Ⅰ型病毒 IgM（捕獲法二步法）    
Rabbit Anti-chicken IgM/Cy5.5        Cy5.5標記的兔抗雞IgM     0.1ml SNAPC3    英文名稱：    SNAPC3蛋白抗體     0.1ml
Rabbit Anti-chicken IgM/Cy7        Cy7標記的兔抗雞IgM     0.1ml Bovine IgM/FITC        熒光素標記牛IgM     0.3ml
Rabbit Anti-chicken IgM/Cy5        Cy5標記的兔抗雞IgM     0.1ml HTRA2    英文名稱：    線粒體*蛋白酶抗體     0.2ml
Rabbit Anti-chicken IgM/PE        PE標記的兔抗雞IgM     0.1ml 賴氨酸缺陷型蛋白激酶1抗體    WNK1    賴氨酸缺陷型蛋白激酶1抗體     0.2ml
Rabbit Anti-dog IgG/RBITC        羅丹明標記的兔抗狗IgG     0.3ml 細胞核分離基因C蛋白抗體    NUDC    細胞核分離基因C蛋白抗體     0.2ml
Rabbit Anti-chicken IgM/APC        APC標記的兔抗雞IgM     0.1ml 2號染色體開放閱讀框54抗體    C2orf54    2號染色體開放閱讀框54抗體     0.2ml
Rabbit Anti-chicken IgM/PE-CY5        PE-CY5標記的兔抗雞IgM     0.1ml 多配體聚糖4抗體    Syndecan 4    多配體聚糖4抗體     0.1ml
Rabbit Anti-chicken IgM/PE-Cy3        PE-Cy3標記的兔抗雞IgM     0.1ml FOS B        FosB抗原     0.5mg
小鼠Ca-ATP酶(Ca-ATPase)ELISA試劑盒   進口分裝   ，英文名：   Ca-ATPase ELISA Kit  Rat Laminin alpha 1,LAMA1 ELISA Kit    大鼠層粘蛋白α1(LAMA1)ELISA Kit
大鼠Tau蛋白ELISA Kit   Rat Tau Proteins ELISA kit  Rat complement factor D(adipsin),CFD ELISA kit    大鼠補體因子D(adipsin)(CFD)ELISA Kit
白介素6受體(IL-6R)ELISA試劑盒   *   ，英文名：   IL-6R ELISA Kit  Rat asymmetrical dimethylarginine,ADMA ELISA Kit    大鼠不對稱二甲基*(ADMA)ELISA Kit
醛脫氫酶1家族成員A1(ALDH1A1)ELISA試劑盒   進口分裝   ，英文名：   ALDH1A1 ELISA Kit  Rat Interleukin 33,IL-33 ELISA Kit    大鼠白介素33(IL-33)ELISA Kit
V-Yes-1Yamaguchi肉瘤病毒相關癌基因同源物(LYN)ELISA試劑盒   進口原裝   ，英文名：   LYN ELISA Kit  Rat Complement fragment 5,C5 ELISA Kit    大鼠補體片斷5(C5)ELISA Kit
綿羊*(cAMP)ELISA Kit   Sheep cyclic adenosine monophosphate,cAMP ELISA Kit  Rat soluble interleukin-2 receptor,sIL-2Rα ELISA kit    大鼠白介素2可溶性受體α鏈(sIL-2Rα/CD25)ELISA Kit
大鼠可溶性凝集素樣氧化低密度脂蛋白受體1(sLOX-1)ELISA Kit   Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1,sLOX-1 ELISA Kit  Rat soluble interleukin-2 receptor,sIL-2Rβ ELISA kit    大鼠白介素2可溶性受體β鏈(sIL-2Rβ)ELISA Kit
防御素β116(DEFβ116)ELISA試劑盒   *   ，英文名：   DEFβ116 ELISA Kit  Rat Interleukin 28A,IL-28A ELISA kit    大鼠白介素28A(IL-28A)ELISA Kit
C-型凝集素域家族4成員K(CLEC4K)ELISA試劑盒   *   ，英文名：   CLEC4K ELISA Kit  Rat Interleukin 26,IL-26 ELISA Kit    大鼠白介素26(IL-26)ELISA Kit
免疫球蛋白A(IgA)ELISA試劑盒   進口原裝   ，英文名：   IgA ELISA Kit  Rat Interleukin 28B,IL-28B ELISA Kit    大鼠白介素28B(IL-28B)ELISA Kit
磷酸烯醇式酸羧激酶(PCK)ELISA試劑盒   進口分裝   ，英文名：   PCK ELISA Kit  Rat Interleukin 22,IL-22 ELISA Kit    大鼠白介素22(IL-22)ELISA Kit
小鼠糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒   進口分裝   ，英文名：   GHbA1c ELISA Kit  Rat soluble interleukin-1 receptor Ⅱ,sIL-1RⅡ ELISA kit    大鼠白介素1可溶性受體Ⅱ(sIL-1RⅡ)ELISA Kit