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TSZelisa試劑盒操作方法:
1.包被:用0.05MPH9.牰碳酸鹽包被緩沖液將抗體稀釋至蛋白質(zhì)含量為1~10μg/ml。在每個(gè)聚苯乙烯板的反應(yīng)孔中加0.1ml,4℃ 。次日,棄去孔內(nèi)溶液,用洗滌緩沖液洗3次,每次3分鐘。(簡稱洗滌,下同)。
2. 小鼠白介素ELISA試劑盒加樣:加一定稀釋的待檢樣品0.1ml于上述已包被之反應(yīng)孔中,置37℃ 孵育1小時(shí)。然后洗滌。(同時(shí)做空白孔,陰性對照孔及陽性對照孔)。
3. 加酶標(biāo)抗體:于各反應(yīng)孔中,加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.1ml。37℃ 孵育0.5~1小時(shí),洗滌。
4. 加底物液顯色:于各反應(yīng)孔中加入臨時(shí)配制的TMB底物溶液0.1ml,37℃ 10~30分鐘。
5. 小鼠白介素ELISA試劑盒終止反應(yīng):于各反應(yīng)孔中加入2M硫酸0.05ml。
6. 結(jié)果判定:可于白色背景上,直接用肉眼觀察結(jié)果:反應(yīng)孔內(nèi)顏色越深,陽性程度越強(qiáng),陰性反應(yīng)為無色或極淺,依據(jù)所呈顏色的深淺,以“+”、“-”號表示。也可測OD值:在ELISA檢測儀上,于450nm(若以ABTS顯色,則410nm)處,以空白對照孔調(diào)零后測各孔OD值,若大于規(guī)定的陰性對照OD值的2.1倍,即為陽性。
TSZelisa試劑盒Human Somatostatin,SS ELISA Kit 人生長抑素(SS)ELISA試劑盒 規(guī)格: 96T/48T
Human sorbitol dehydrogenase,SDH ELISA Kit 人山梨醇脫氫酶(SDH)ELISA試劑盒 規(guī)格: 96T/48T
Human Specifie Macrophage Arming Paetot,SMAF ELISA Kit 人特異性巨噬細(xì)胞武裝因子(SMAF)ELISA試劑盒 規(guī)格: 96T/48T
Human sphingomyelin,SM ELISA Kit 人鞘磷脂(SM)ELISA試劑盒 規(guī)格: 96T/48T
Human spleen tyrosine kinase,Syk ELISA Kit 人脾臟酪氨酸激酶(Syk)ELISA試劑盒 規(guī)格: 96T/48T
Human Squamous Cell Carcinoma Antigen 2,SCCAg 2 ELISA Kit 人鱗狀上皮細(xì)胞癌抗原2(SCCAg-2)ELISA試劑盒 規(guī)格: 96T/48T
Human squamous cell carcinoma related antigen,SCCAg ELISA Kit 人鱗狀細(xì)胞癌相關(guān)抗原(SCCAg)ELISA試劑盒 規(guī)格: 96T/48T
Human sreum albumin,HSA ELISA Kit 人血清白蛋白(HSA)ELISA試劑盒 規(guī)格: 96T/48T
Human SS-B/La Antibody,ELISA Kit 人抗SS-B/La抗體ELISA試劑盒 規(guī)格: 96T/48T
Human Staphylococal protein A,SPA ELISA Kit 人葡萄球菌蛋白A(SPA)ELISA試劑盒 規(guī)格: 96T/48T
Human staphylococcus aureus enterotoxins,SE ELISA Kit 人金葡菌腸毒素(SE)ELISA試劑盒 規(guī)格: 96T/48T
Human Stem Cell Factor Receptor,SCFR ELISA Kit 人干細(xì)胞因子受體(SCFR)ELISA試劑盒 規(guī)格: 96T/48T
Human Stem cell factor/mast cell growth factor,SCF/MGF ELISA Kit 人干細(xì)胞因子/肥大細(xì)胞生長因子(SCF/MGF)ELISA試劑盒 規(guī)格: 96T/48T
Human steroid producing cell autoantibody,SCA ELISA Kit 人抗類固醇生成細(xì)胞抗體(SCA)ELISA試劑盒 規(guī)格: 96T/48T
Human Streptokinase,SK ELISA Kit 人鏈激酶(SK)ELISA試劑盒 規(guī)格: 96T/48T
Human Stromal cell derived factor 1a,SDF-1a ELISA Kit 人基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒 規(guī)格: 96T/48T
Human Stromal cell derived factor 1β,SDF-1β ELISA Kit 人基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)ELISA試劑盒 規(guī)格: 96T/48T
Human Stromelysin-1,ST1 ELISA Kit 人基質(zhì)裂解素(ST1)ELISA試劑盒 規(guī)格: 96T/48T
Human Stromelysin-2,ST2 ELISA Kit 人基質(zhì)裂解素(ST2)ELISA試劑盒 規(guī)格: 96T/48T
Human Stromelysin-3,ST3 ELISA Kit 人基質(zhì)裂解素(ST3)ELISA試劑盒 規(guī)格: 96T/48T
Human substance P receptor,SP-R ELISA Kit 人P物質(zhì)受體(SP-R)ELISA試劑盒 規(guī)格: 96T/48T
Human Super Oxidase Dimutase,SOD ELISA Kit 人超氧化物歧化酶(SOD)ELISA試劑盒 規(guī)格: 96T/48T
Human Surface Membrane Immunoglobulin,SmIg ELISA Kit 人細(xì)胞膜表面免疫球蛋白(SmIg)ELISA試劑盒 規(guī)格: 96T/48T
Human Surfactant Protein A,SP-A ELISA Kit 人表面活性蛋白A(SP-A)ELISA試劑盒 規(guī)格: 96T/48T
Human Surfactant Protein D,SP-D ELISA Kit 人表面活性蛋白D(SP-D)ELISA試劑盒 規(guī)格: 96T/48T
Human systemic lupus erythematosus,SLE ELISA Kit 人系統(tǒng)性紅斑狼瘡(SLE)ELISA試劑盒 規(guī)格: 96T/48T
Human T cell receptor,TCR ELISA Kit 人T細(xì)胞受體(TCR)ELISA試劑盒 規(guī)格: 96T/48T
Human Tacrolimus-FK506 ELISA Kit 人他克莫司(FK506)ELISA試劑盒 規(guī)格: 96T/48T
Human talin ELISA Kit 人踝蛋白(talin)ELISA試劑盒 規(guī)格: 96T/48T
Human targeted ribonuclease, TR ELISA Kit 人靶向核糖核酸酶(TR)ELISA試劑盒 規(guī)格: 96T/48T
Human tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit 人抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA試劑盒 規(guī)格: 96T/48T
Human T-cell acute lymphoblastic leukemia antigen,TALLA-1 ELISA Kit 人T細(xì)胞急性淋巴母細(xì)胞白血病相關(guān)抗原(TALLA-1/CD231)ELISA試劑盒 規(guī)格: 96T/48T
Human T-cell specific GTPase,Tgtp ELISA Kit 人T細(xì)胞特異性鳥苷三磷酸酶(Tgtp)ELISA試劑盒 規(guī)格: 96T/48T
Human omerase,TE ELISA Kit 人端粒酶(TE)ELISA試劑盒 規(guī)格: 96T/48T
Human tenascin-R,TN-R ELISA Kit 人肌腱蛋白R(TN-R)ELISA試劑盒 規(guī)格: 96T/48T
Human terminal complement complex C5b-9,TCC C5b-9 ELISA Kit 人末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)ELISA試劑盒 規(guī)格: 96T/48T
Human terminal deoxynucleotidyl transferase,TdT ELISA Kit 人末端脫氧核苷酸轉(zhuǎn)移酶(TdT)ELISA試劑盒 規(guī)格: 96T/48T
Human Testoterone recepter,TR ELISA Kit 人睪酮受體(TR)ELISA試劑盒 規(guī)格: 96T/48T
Human Testoterone,T ELISA Kit 人睪酮(T)ELISA試劑盒 規(guī)格: 96T/48T
Human Tetanus Antibody ELISA Kit 人破傷風(fēng)抗體(Tetanus Ab)ELISA試劑盒 規(guī)格: 96T/48T
Human TGF-beta-inducible early response gene-1,TIEG1 ELISA Kit 人TGF-β誘導(dǎo)早期基因1(TIEG1)ELISA試劑盒 規(guī)格: 96T/48T
Human T-H glycoprotein,THP ELISA Kit 人TH糖蛋白(THP)ELISA試劑盒 規(guī)格: 96T/48T
Human thioredoxin reductase,TrxR ELISA Kit 人硫氧還蛋白還原酶(TrxR)ELISA試劑盒 規(guī)格: 96T/48T
Human Thioredoxin,Trx ELISA Kit 人硫氧化還原蛋白(Trx)ELISA試劑盒 規(guī)格: 96T/48T
Human Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit 人纖溶抑制因子/凝血酶激活的纖溶抑制物(TAFI)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombin receptor,TR ELISA Kit 人凝血酶受體(TR)ELISA試劑盒 規(guī)格: 96T/48T
Human Thrombin,TM ELISA Kit 人凝血酶(TM)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombin-antithrombin complex,TAT ELISA Kit 人凝血酶抗凝血酶復(fù)合物(TAT)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombinthrombomodulin compound,T-TM ELISA Kit 人凝血酶血栓調(diào)節(jié)蛋白復(fù)合物(T-TM)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombomodulin,TM ELISA Kit 人血栓調(diào)節(jié)蛋白(TM)ELISA試劑盒 規(guī)格: 96T/48T
Human Thrombopoietin,TPO ELISA Kit 人血小板生成素(TPO)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombospondin 1,TSP-1 ELISA Kit 人血小板反應(yīng)蛋白/凝血酶敏感蛋白1(TSP-1)ELISA試劑盒 規(guī)格: 96T/48T
Human Thromboxane A2,TX-A2 ELISA Kit 人血栓素A2(TX-A2)ELISA試劑盒 規(guī)格: 96T/48T
Human Thromboxane B2,TX-B2 ELISA Kit 人血栓素B2(TXB2)ELISA試劑盒 規(guī)格: 96T/48T
Human thrombus precursor protein,TpP ELISA Kit 人血栓前體蛋白(TpP)ELISA試劑盒 規(guī)格: 96T/48T
Human thymic stromal lymphopoietin,TSLP ELISA Kit 人胸腺基質(zhì)淋巴細(xì)胞生成素(TSLP)ELISA試劑盒 規(guī)格: 96T/48T
Human thymidinephosphorylase,TP ELISA Kit 人胸腺嘧啶核苷磷酸化酶(TP)ELISA試劑盒 規(guī)格: 96T/48T
Human thymidylate synthetase,TS ELISA Kit 人胸苷酸合成酶(TS)ELISA試劑盒 規(guī)格: 96T/48T
Human Thymopentin,TP-5 ELISA Kit 人胸腺五肽(TP-5)ELISA試劑盒 規(guī)格: 96T/48TTSZelisa試劑盒
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