背景介紹：NDC1 is a 674 amino acid multi-pass membrane protein, central core structure of the nuclear pore complex (NPC) and member of the NDC1 family that is crucial for selective nuclear protein import. Existing as four alternatively spliced isoforms that are encoded by a gene located on human chromosome 1, NDC1 interacts with Nup35 and anchors Aladin to the nuclear envelope of the NPC, a region of macromolecular transport between the nucleus and cytoplasm. In the absence of NDC1, Aladin becomes mislocalized and may lead to the development of an autosomal recessive disorder termed achalasia-addisonianism-alacrima (triple A) syndrome. Triple A syndrome is characterized by achalasia, alacrima and adrenocortico-tropin-resistant adrenal insufficiency. Robust expression in neural systems associated with cognitive, motor and sensory functions is consistent with the myriad of symptoms experienced by patients with triple A syndrome.

別名：nucleoporin NDC1; TMEM48; transmembrane protein 48; NET3.

跨膜蛋白48抗體，Anti-NDC1抗體簡單介紹：

產(chǎn)品編號(hào)：BYK-19057R

英文名稱：Anti-NDC1
中文名稱：跨膜蛋白48抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

跨膜蛋白48抗體，Anti-NDC1抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對(duì)應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

血清封閉，減少非特異性結(jié)合

組織中非抗原抗體反應(yīng)出現(xiàn)的陽性染色稱為非特異性背景染色，zui常見的原因是蛋白吸附于高電荷的膠原和結(jié)締組織成分上。zui有效方法是在用*抗體前加與制備第二抗體相同動(dòng)物的非免疫血清（1:5-1:20），封閉組織上帶電荷基團(tuán)而除去與一抗非特異性結(jié)合。必要時(shí)加入2%-5%牛血清白蛋白，可進(jìn)一步減少非特異性染色，作用時(shí)間為10-20min。免疫熒光染色時(shí)，可用0.01%伊文藍(lán)（PBS溶液）稀釋熒光抗體，對(duì)消除背景的非特異性熒光染色有很好的效果。洗滌用的緩沖液中加入0.85%-1%NaCl成為高鹽溶液，充分洗滌切片，能有效的減少非特異性結(jié)合而減少背景染色。

跨膜蛋白48抗體，Anti-NDC1抗體相關(guān)產(chǎn)品：

Anti-NSBP1 核小體結(jié)合蛋白1抗體
Anti-NRSF/REST 神經(jīng)元抑制蛋白抗體
Anti-Neuropilin-2/NRP2 神經(jīng)纖毛蛋白2抗體
Anti-NRP1/CD304 神經(jīng)纖毛蛋白1抗體
Anti-CD303/BDCA-2 C型凝集素結(jié)構(gòu)域家族4成員C抗體
Anti-BAF53A 肌動(dòng)蛋白樣蛋白6A抗體
Anti-NRF-1 核呼吸因子-1抗體
Anti-CPLX2 Complexin II/復(fù)合素2抗體
Anti-Nrf2 核因子2相關(guān)因子2抗體
Anti-CNTN4/AXCAM  軸突相關(guān)粘附分子抗體
Anti-PCDHGA3 原鈣粘蛋白γA3抗體
Anti-NRAS 原癌基因N-Ras抗體
Anti-NR2D/NMDAR2D  *受體2D抗體