背景介紹：The mesoderm induction early response (MIER) protein family (also known as the fibroblast growth factor (FGF)-regulated immediate-early protein family) comprises a group of proteins that are activated by FGF (fibroblast growth factor). This suggests that MEIR proteins may play a significant role in FGF-regulated cellular activities and in the progression of certain cancers. MIER proteins contain one SANT domain, which is involved in transcriptional activation and repression, and one ELM2 domain, which was first characterized in egl-27, a gene that is critically involved in embryonic patterning of C. elegans. MIER1, formerly known as early response 1 (ER1), was first cloned and characterized in Xenopus. Expression of MIER1 is negligible in most normal tissues, but has been found to be upregulated in breast carcinoma cell lines and tumors. MIER1 functions as a transcriptional repressor of a number of genes including Sp1 target genes, most likely through interaction with HDAC1.

別名：DKFZp686L09111; DKFZp781G1119; DKFZP781I1119; FLJ35954; Mesoderm induction early response 1 family member 3; Mesoderm induction early response protein 3; Mi-er3; MIER 3; MIER3; MIER3_HUMAN. 

中胚層誘導(dǎo)早期反應(yīng)蛋白3抗體,MIER3抗體簡單介紹：

產(chǎn)品編號(hào)：BYK-18939R

英文名稱：Anti-MIER3
中文名稱：中胚層誘導(dǎo)早期反應(yīng)蛋白3抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

中胚層誘導(dǎo)早期反應(yīng)蛋白3抗體,MIER3抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對(duì)應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

流式檢測樣品的制備

提示

1、流式細(xì)胞儀檢測需要將細(xì)胞制備成單細(xì)胞懸液。

2、避免細(xì)胞成團(tuán)，以免堵塞流式細(xì)胞儀。

3、實(shí)體組織制備單細(xì)胞懸液需要物理方法分離或酶消化法進(jìn)行，具體組織的方法，依照經(jīng)驗(yàn)來進(jìn)行。

方案A: 培養(yǎng)細(xì)胞的制備

實(shí)驗(yàn)材料：0.25%胰，15ml或50ml的離心管。

實(shí)驗(yàn)流程：

1、如果是懸浮細(xì)胞，則小心將細(xì)胞移入離心管中，進(jìn)行計(jì)數(shù)和活力分析。進(jìn)行步驟3。

2、如果是貼壁細(xì)胞，建議使用0.25%的*消化細(xì)胞，制備成單細(xì)胞懸液后置于離心管中計(jì)數(shù)和活力分析。

3、200g 離心5min。棄去上清，應(yīng)用適量的流式孵育緩沖液重懸細(xì)胞，調(diào)整細(xì)胞濃度為1×107/ml。

方案B: 組織細(xì)胞制備。

實(shí)驗(yàn)材料與試劑：剪刀和鑷子，載玻片， 60×15mm的組織培養(yǎng)皿；細(xì)胞過濾器（尼龍網(wǎng)過濾）,0.01MPBS。

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