背景介紹：JRAB is a 904 amino acid protein with one CH (calponin-homology) domain and one LIM zinc-binding domain. JRAB has been shown to interact with Rab13 and Rab8 to facilitate cellular transport of claudin-1, occludin, and E-cadherin. This interaction is vital for the coordination of the assembly of tight junctions (TJs) and adherens junctions (AJs). Dynamic turnover (endocytic recycling) of cell-to-cell AJs and TJs is essential for epithelial morphogenesis during normal development and differentiation. The endocytic recycling of occludin and cluadin proteins is part of an ongoing process of restructuring and maintaining cell junctions, especially at TJs. JRAb and Rab13 have also been implicated in the carcinoma metastasis event of epithelial cell scattering. This event shows Rab13 and JRAB colocalizing with F-Actin in lamellipodial structures prior to cell scattering.

別名：FLJ23471; FLJ41996; FLJ44858; FLJ45410; JRAB; Junctional Rab13 binding protein; MGC46023; MICAL L2; MICAL like 2; MICAL like protein 2; MICALL2. 

MICAL樣蛋白2抗體,Anti-MICALL2抗體簡單介紹：

產(chǎn)品編號：BYK-18936R

英文名稱：Anti-MICALL2
中文名稱：MICAL樣蛋白2抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

MICAL樣蛋白2抗體,Anti-MICALL2抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

流式檢測樣品的制備

提示

1、流式細(xì)胞儀檢測需要將細(xì)胞制備成單細(xì)胞懸液。

2、避免細(xì)胞成團(tuán)，以免堵塞流式細(xì)胞儀。

3、實(shí)體組織制備單細(xì)胞懸液需要物理方法分離或酶消化法進(jìn)行，具體組織的方法，依照經(jīng)驗(yàn)來進(jìn)行。

方案A: 培養(yǎng)細(xì)胞的制備

實(shí)驗(yàn)材料：0.25%胰，15ml或50ml的離心管。

實(shí)驗(yàn)流程：

1、如果是懸浮細(xì)胞，則小心將細(xì)胞移入離心管中，進(jìn)行計(jì)數(shù)和活力分析。進(jìn)行步驟3。

2、如果是貼壁細(xì)胞，建議使用0.25%的*消化細(xì)胞，制備成單細(xì)胞懸液后置于離心管中計(jì)數(shù)和活力分析。

3、200g 離心5min。棄去上清，應(yīng)用適量的流式孵育緩沖液重懸細(xì)胞，調(diào)整細(xì)胞濃度為1×107/ml。

方案B: 組織細(xì)胞制備。

實(shí)驗(yàn)材料與試劑：剪刀和鑷子，載玻片， 60×15mm的組織培養(yǎng)皿；細(xì)胞過濾器（尼龍網(wǎng)過濾）,0.01MPBS。

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