背景介紹：DHRS11 is a 260 amino acid secreted protein. Belonging to the short-chain dehydrogenases/reductases (SDR) family, DHRS11 forms a homotetramer and is expressed as two isoforms produced by alternative splicing events. The gene that encodes DHRS11 maps to human chromosome 17, which makes up over 2.5% of the human genome with about 81 million bases encoding over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. Chromosome 17 is also linked to neurofibromatosis, a condition characterized by neural and epidermal lesions, and dysregulated Schwann cell growth. Alexander disease, Birt-Hogg-Dube syndrome and Canavan disease are also associated with chromosome 17. 

別名：MGC4172; ARPG836; Dehydrogenase/reductase (SDR family) member 11; Dehydrogenase/reductase SDR family member 11; Dehydrogenase/reductase SDR family member 11 precursor; DHR11_HUMAN; DHRS11; FLJ39232; SDR24C1; short chain dehydrogenase/reductase; Short chain dehydrogenase/reductase family 24C, member 1.  

DHRS11蛋白抗體/Anti-DHRS11抗體簡單介紹：

產(chǎn)品編號：BYK-18921R

英文名稱：Anti-DHRS11
中文名稱：膠質(zhì)瘤發(fā)病相關(guān)蛋白1樣蛋白2抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

DHRS11蛋白抗體/Anti-DHRS11抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對應(yīng)熒光素的選擇：

藍(lán)色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

流式檢測樣品的制備

提示

1、流式細(xì)胞儀檢測需要將細(xì)胞制備成單細(xì)胞懸液。

2、避免細(xì)胞成團(tuán)，以免堵塞流式細(xì)胞儀。

3、實(shí)體組織制備單細(xì)胞懸液需要物理方法分離或酶消化法進(jìn)行，具體組織的方法，依照經(jīng)驗(yàn)來進(jìn)行。

方案A: 培養(yǎng)細(xì)胞的制備

實(shí)驗(yàn)材料：0.25%胰，15ml或50ml的離心管。

實(shí)驗(yàn)流程：

1、如果是懸浮細(xì)胞，則小心將細(xì)胞移入離心管中，進(jìn)行計(jì)數(shù)和活力分析。進(jìn)行步驟3。

2、如果是貼壁細(xì)胞，建議使用0.25%的*消化細(xì)胞，制備成單細(xì)胞懸液后置于離心管中計(jì)數(shù)和活力分析。

3、200g 離心5min。棄去上清，應(yīng)用適量的流式孵育緩沖液重懸細(xì)胞，調(diào)整細(xì)胞濃度為1×107/ml。

方案B: 組織細(xì)胞制備。

實(shí)驗(yàn)材料與試劑：剪刀和鑷子，載玻片， 60×15mm的組織培養(yǎng)皿；細(xì)胞過濾器（尼龍網(wǎng)過濾）,0.01MPBS。

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