背景介紹：L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene.

別名：FLJ40498; GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87; GLUR7; Glutamate receptor metabotropic 7; GPRC1G; GRM7; GRM7_HUMAN; Metabotropic glutamate receptor 7; mGluR7; OTTHUMP00000206961; OTTHUMP00000214674; OTTHUMP00000214675.  

促代謝型*受體7抗體,Anti-mGluR7抗體簡單介紹：

產(chǎn)品編號：BYK-18803R

英文名稱：Anti-mGluR7
中文名稱：促代謝型*受體7抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

促代謝型*受體7抗體,Anti-mGluR7抗體產(chǎn)品信息：

博研生物小課堂：

常用激光器對應(yīng)熒光素的選擇：

藍色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

胞內(nèi)抗體間接標記

1、將消化下來的細胞用1 X PBS洗兩遍，然后用0.5 mL 0.5%BSA的 1 X PBS重懸。

2、加入等量預(yù)熱的4%多聚甲醛固定細胞，37℃孵育10分鐘。

3、用0.5%BSA的 1 X PBS洗兩遍并用1ml 90%的冰甲醇重懸細胞，放置冰上30分鐘。

4、用0.5%BSA的 1 X PBS洗兩遍。

5、樣本標記：100μl 0.5%BSA的 1 X PBS內(nèi)細胞量為5X105-1X106個。

a)不標記細胞樣本

b)單獨二抗

c)同型對照+二抗

d)一抗+二抗

6、以抗體說明書上的比例加入抗體，冰上孵育30分鐘。

7、用0.5%BSA的 1 X PBS洗兩遍。

8、0.5%BSA的 1 X PBS重懸細胞后加入等體積10%山羊血清，室溫孵育15分鐘。

9、加入熒光標記二抗，放置冰上，避光條件下孵育30分鐘。

10、用0.5%BSA的1 X PBS洗兩遍。

11、加入500μl 1 X PBS上機檢測。

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