背景介紹：MAGE-H1 is a 219 amino acid protein that contains a type II MAGE homology domain (MHD). Enhanced ligand stimulation promotes MAGE-H1 interaction with the type II death domain of NGFR p75. It is suggested that MAGE-H1 accelerates differentiation in response to nerve growth factor in cells.

Function:
Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.

別名：dJ730K3.2; MAGI-3; magi3; MAGI3_HUMAN; Membrane Associated Guanylate kinase Related 3; Membrane-associated guanylate kinase; Membrane-associated guanylate kinase inverted 3; RP4-730K3.1; SLIPR; WW and PDZ domain-containing protein 3.  

膜相關(guān)鳥苷酸激酶3抗體,Anti-MAGI3抗體簡單介紹：

產(chǎn)品編號：BYK-18629R

英文名稱：Anti-MAGI3
中文名稱：膜相關(guān)鳥苷酸激酶3抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

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性    狀 Lyophilized or Liquid
濃    度 1mg/ml

膜相關(guān)鳥苷酸激酶3抗體,Anti-MAGI3抗體產(chǎn)品信息：

免疫組化問題解答

染色弱？

原因：抗體濃度過低、孵育時間過短

解決方法：提高抗體濃度、孵育時間不能少于60分鐘  

原因：試劑超過有效使用時間 

解決方法：應(yīng)更換試劑 

原因：操作中添加試劑時緩沖液未瀝干 

解決方法：每步滴加試劑前瀝干切片中多余的緩沖液 使試劑稀釋 （應(yīng)防止切片干燥） 

原因：室溫太低，低于15度 

解決方法：要改放在37度孵育箱孵育30-60分鐘或4度冰箱過夜 

原因：蛋白封閉過度 

解決方法：封閉不要超過12分鐘

染色陰性？

原因：操作步驟錯誤

解決方法：應(yīng)重試，設(shè)陽性對照

原因：組織中無抗原

解決方法：設(shè)陽性對照以驗(yàn)證試驗(yàn)結(jié)果

原因：一抗與二抗種屬連接錯誤

解決方法：仔細(xì)確定一抗二抗種屬無誤

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