背景介紹：The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene.

別名：Avian musculoaponeurotic fibrosarcoma virus (v maf) AS42 oncogene, protein F; CTA 447C4.1; hMafF; MAFF; MAFF_HUMAN; OTTHUMP00000199299; Transcription factor MafF; U MAF; U-Maf; V maf avian musculoaponeurotic fibrosarcoma oncogene family, protein F; V maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian); V maf musculoaponeurotic fibrosarcoma oncogene homolog F; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F. 

轉(zhuǎn)錄因子MAFF抗體,Anti-MAFF抗體簡(jiǎn)單介紹：

產(chǎn)品編號(hào)：BYK-18614R

英文名稱(chēng)：Anti-MAF1
中文名稱(chēng)：轉(zhuǎn)錄因子MAFF抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來(lái)源 Rabbit or Mouse

克隆類(lèi)型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請(qǐng)或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

轉(zhuǎn)錄因子MAFF抗體,Anti-MAFF抗體產(chǎn)品信息：

免疫組化問(wèn)題解答

染色弱？

原因：抗體濃度過(guò)低、孵育時(shí)間過(guò)短

解決方法：提高抗體濃度、孵育時(shí)間不能少于60分鐘  

原因：試劑超過(guò)有效使用時(shí)間 

解決方法：應(yīng)更換試劑 

原因：操作中添加試劑時(shí)緩沖液未瀝干 

解決方法：每步滴加試劑前瀝干切片中多余的緩沖液 使試劑稀釋 （應(yīng)防止切片干燥） 

原因：室溫太低，低于15度 

解決方法：要改放在37度孵育箱孵育30-60分鐘或4度冰箱過(guò)夜 

原因：蛋白封閉過(guò)度 

解決方法：封閉不要超過(guò)12分鐘

染色陰性？

原因：操作步驟錯(cuò)誤

解決方法：應(yīng)重試，設(shè)陽(yáng)性對(duì)照

原因：組織中無(wú)抗原

解決方法：設(shè)陽(yáng)性對(duì)照以驗(yàn)證試驗(yàn)結(jié)果

原因：一抗與二抗種屬連接錯(cuò)誤

解決方法：仔細(xì)確定一抗二抗種屬無(wú)誤

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