背景介紹：Ly-6D is a 128 amino acid glycoprotein that is expressed in squamous cell carcinoma cell lines and squamous cell epithelia tissue. Ly-6D contains a signal peptide, 2 theoretical phosphorylation sites and 3 putative myristoylation sites. Upregulation of the gene encoding Ly-6D in head and neck cancers is associated with poor prognosis and high expression of Ly-6D has been linked to enhanced cell migration. Ly-6D is frequently used as a molecular marker for diagnosis and therapy of head-and-neck squamous cell carcinoma (HNSCC). It has been suggested that Ly-6D may regulate the expression levels of certain fucosylated E-selectin ligands and protein FX, a protein that contributes to the last step in the synthesis of GDP-L-fucose, in HNSCC cell lines. This finding is indicative that Ly-6D may regulate tumor cell adhesion in inflamed vessel walls that express E-selectin.

別名：E48 antigen; LY 6D; LY-6D;Lymphocyte antigen 6 complex; LY6D_HUMAN; Lymphocyte antigen 6 complex locus D; Lymphocyte antigen D; Lymphocyte antigen 6D. 

淋巴細(xì)胞抗原Ly6D抗體,Anti-LY6D抗體簡單介紹：

產(chǎn)品編號：BYK-18604R

英文名稱：Anti-LY6D
中文名稱：淋巴細(xì)胞抗原Ly6D抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

淋巴細(xì)胞抗原Ly6D抗體,Anti-LY6D抗體產(chǎn)品信息：

免疫組化問題解答

染色過強(qiáng)？

原因：抗體濃度過高或孵育時(shí)間過長

解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過夜

原因：孵育溫度過高超過37度:

解決方法：一般在室溫20-28度

原因：DAB顯色時(shí)間過長或濃度過高?

解決方法：顯色時(shí)間不超過5-12分鐘，以顯微鏡下觀察為準(zhǔn)

非特異性背景染色？

原因：操作過程中沖洗不充分

解決方法：每步?jīng)_洗3次每次5分鐘

原因：組織中含過氧化物酶未阻斷 

解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長 

原因：組織中含內(nèi)原性* 

解決方法：正常非免疫動物血清再封閉

原因：血清蛋白封閉不充分 

解決方法：血清蛋白封閉不充分

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