背景介紹：This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood

別名：Pyruvate Dehydrogenase E2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; anti DLAT; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide; Dihydrolipoamide S Acetyltransferase; Dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex); Dihydrolipoamide S-Acetyltransferase; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; DLAT; DLTA; E2; E2 component of pyruvate dehydrogenase complex; EC 2.3.1.12; M2 antigen complex 70 kDa subunit; M2 Antigen Complex 70kD Subunit; mitochondrial; ODP2_HUMAN; PBC; PDC E2; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; Pyruvate dehydrogenase complex E2 subunit; S acetyltransferase component of pyruvate dehydrogenase complex. 

丙酮酸脫氫酶E2抗體,Anti-PDCE2抗體簡單介紹：

產(chǎn)品編號：BYK-19695R

英文名稱：Anti-PDCE2
中文名稱：丙酮酸脫氫酶E2抗體

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

丙酮酸脫氫酶E2抗體,Anti-PDCE2抗體產(chǎn)品信息：

Western Blotting 具體實驗步驟

我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜（二氟化樹脂膜膜孔徑：0.45um)也可以使用，但硝酸纖維素膜比較脆，在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。

通常如果使用Bio-Rad的標準濕式轉(zhuǎn)膜裝置，可以設(shè)定轉(zhuǎn)膜電壓120V，轉(zhuǎn)膜時間為30-60分鐘。具體的轉(zhuǎn)膜時間要根據(jù)目的蛋白的大小而定，目的蛋白的分子量越大，需要的轉(zhuǎn)膜時間越長，目的蛋白的分子量越小，需要的轉(zhuǎn)膜時間越短。

在轉(zhuǎn)膜過程中，特別是高電流快速轉(zhuǎn)膜時，通常會有非常嚴重的發(fā)熱現(xiàn)象，把轉(zhuǎn)膜槽放置在冰浴中進行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預染蛋白質(zhì)分子量標準，通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進行染色，以觀察實際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進行染色，以觀察蛋白的殘留情況。

丙酮酸脫氫酶E2抗體,Anti-PDCE2抗體相關(guān)產(chǎn)品：

Anti-RTP3 受體轉(zhuǎn)運蛋白3抗體
Anti-SNAI2/SLUG 鋅指轉(zhuǎn)錄因子Slug抗體
Anti-A4GNT α1,4-N-乙酰葡糖胺轉(zhuǎn)移酶α4Gn-T抗體
Anti-LIV-1/SLC39A6 雌激素調(diào)節(jié)蛋白LIV1/鋅轉(zhuǎn)運蛋白抗體
Anti-NELL2/NRP2 蛋白激酶C結(jié)合蛋白NELL2抗體
Anti-SLT/SLT-IIe/O139 豬水腫素(O139)菌體蛋白抗體
Anti-GODZ/ZNF373 棕櫚酰轉(zhuǎn)移酶GODZ抗體
Anti-CRIM1 富含半*運動神經(jīng)元蛋白1抗體
Anti-SLC34A2 磷酸鈉協(xié)同轉(zhuǎn)運蛋白抗體
Anti-SLC33A1 乙酰*轉(zhuǎn)運蛋白1抗體
Anti-Smac/DIABLO 線粒體促凋亡蛋白抗體