背景介紹：DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/*; in vitro, stimulates dissociation of * from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

別名：100 kDa DNA pairing protein; 100 kDa DNA-pairing protein; 100 kDa subunit; DNA binding p52/p100 complex 100 kDa subunit; DNA-binding p52/p100 complex; hPOMp100; Polypyrimidine tract binding protein associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; proline- and glutamine-rich; PSF; PTB associated splicing factor; PTB-associated-splicing factor; Sfpq; SFPQ_HUMAN; Splicing factor; Splicing factor proline and glutamine rich; Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated).  

多聚嘧啶序列結(jié)合蛋白相關(guān)剪接因子PSF抗體簡單介紹：

產(chǎn)品編號：BYK-19683R

英文名稱：Anti-SFPQ
中文名稱：多聚嘧啶序列結(jié)合蛋白相關(guān)剪接因子

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索?。?/span>

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

多聚嘧啶序列結(jié)合蛋白相關(guān)剪接因子PSF抗體產(chǎn)品信息：

Western Blotting 問題解答

背景高？

原因：

1.HRP濃度過高；

2.封閉無效；

3.不適合的封閉試劑；

4.洗滌不充分和洗滌液的量；

5.膠片曝光過度；

6.抗原或抗體的濃度太高。

解決：

1.稀釋HRP至少10倍；

2.尋找合適的封閉方法；

3.選用其他的封閉試劑；

4.增加洗滌的時間,次數(shù)；

5.減少曝光時間；

6.稀釋抗原或抗體。

7、蛋白質(zhì)條帶上有空泡影像？

原因：

1.蛋白質(zhì)沒有被充分轉(zhuǎn)??；

2.膜沒有預(yù)先浸濕；

3.在膠片和膜之間有氣泡。

解決：

1.尋找合適的轉(zhuǎn)移方法和條件；

2.在轉(zhuǎn)印前充分浸濕；

3.在暴光之前趕走氣泡。

多聚嘧啶序列結(jié)合蛋白相關(guān)剪接因子PSF抗體相關(guān)產(chǎn)品：

Anti-Sec8 胞吐分泌蛋白Sec8抗體
Anti-SSTR3 *受體3抗體
Anti-SSTR2 *受體2抗體
Anti-PCDHB4 原鈣粘蛋白β4抗體
Anti-SSTR1 *受體1抗體
Anti-IL-28R 白細(xì)胞介素28受體抗體
Anti-SH3GL3 胞漿蛋白SH3GL3抗體
Anti-CD15/Fut4/SSEA-1 階段特異性胚胎表面抗原-1抗體
Anti-AKAP12/Gravin *抑制蛋白激酶C底物抗體
Anti-A2LD1/GGACT A2LD1蛋白抗體
Anti-SSB/La 干燥癥SSB/La抗體
Anti-PIK3 gamma 磷脂酰肌醇激酶PIK3-γ抗體
Anti-SSA/RO52 核糖核蛋白抗原抗體
Anti-SRG11/Spata17抗體
Anti-NFIB/NF1B2 核因子1B抗體