Anti-INTS2抗體；INTS2蛋白抗體簡單介紹：

產(chǎn)品編號：BYK-18176R

英文名稱：Anti-INTS2
中文名稱：INTS2蛋白抗體

別名：INT2; Integrator complex subunit 2; KIAA1287. INT2_HUMAN 

背景介紹：INTS2 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]). INTS2 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

Anti-INTS2抗體；INTS2蛋白抗體產(chǎn)品信息：

SABC法

- 脫蠟、水化

- PBS洗2次各5分鐘

- 用蒸餾水或PBS配制新鮮的3% H2O2 ，室溫封閉5-10分鐘，用蒸餾水洗3次

- 抗原修復(fù)

- PBS洗5分鐘

- 滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體

- 滴加Ι抗50ul,室溫靜置1小時或4℃過夜或37℃1小時

- PBS洗3次各2分鐘

- 滴加*化Ⅱ抗，20℃-37℃ 20分鐘

- PBS洗3次各2分鐘

- 滴加試劑SABC 20℃-30℃ 20分鐘

- PBS洗4次各5分鐘

- DAB顯色：試劑盒或自配顯色劑顯色

- 脫水、透明、封片、鏡檢

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