Anti-CD101抗體；CD101抗體簡單介紹：

產(chǎn)品編號：BYK-10727R

英文名稱：Anti-CD101
中文名稱：CD101抗體

別名：IGSF2; Immunoglobulin superfamily member 2; Leukocyte surface protein; V7; V7 LSB; IGSF2_HUMAN; IgSF2; Cell surface glycoprotein V7; Glu-Trp-Ile EWI motif-containing protein 101; EWI-101; CD101.  

背景介紹：CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from plaets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. 

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

Anti-CD101抗體；CD101抗體產(chǎn)品信息：

SABC法

- 脫蠟、水化

- PBS洗2次各5分鐘

- 用蒸餾水或PBS配制新鮮的3% H2O2 ，室溫封閉5-10分鐘，用蒸餾水洗3次

- 抗原修復(fù)

- PBS洗5分鐘

- 滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體

- 滴加Ι抗50ul,室溫靜置1小時(shí)或4℃過夜或37℃1小時(shí)

- PBS洗3次各2分鐘

- 滴加*化Ⅱ抗，20℃-37℃ 20分鐘

- PBS洗3次各2分鐘

- 滴加試劑SABC 20℃-30℃ 20分鐘

- PBS洗4次各5分鐘

- DAB顯色：試劑盒或自配顯色劑顯色

- 脫水、透明、封片、鏡檢

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