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The NEXTFLEX^® PCR-Free DNA Sequencing kit is designed to prepare gel-free libraries using magnetic bead-based size selection from as little as 500 ng of input DNA. Amplifying AT or GC rich genomic regions often leads to sequence biased nucleotide compositions and poses a serious challenge during analysis. Using specially designed master-mixed enzymes, the NEXTFLEX^® PCR-Free DNA Library Prep Kit completely eliminates the need for amplification, enabling better read mapping and a reduction in duplicate sequences, leading to reduced sequence cost and bias.

Learn more about the NEXTFLEX^® PCR-Free DNA Library Prep kit