簡(jiǎn)介 Q-Box AQUA采用間歇流動(dòng)呼吸法,測(cè)量水生動(dòng)物(脊椎動(dòng)物和無(wú)脊椎動(dòng)物均可)的代謝速率。即將水生動(dòng)物放置在一個(gè)呼吸室中,通過(guò)溶解氧的減少來(lái)衡量代謝速率。耗氧率(VO2)通過(guò)軟件進(jìn)行計(jì)算,原始溶解氧數(shù)據(jù)經(jīng)鹽度、溫度和壓力校正,計(jì)算結(jié)果顯示并保存在軟件中。 |
優(yōu)點(diǎn) l 間歇流動(dòng)呼吸法無(wú)需將動(dòng)物從試驗(yàn)箱中取出,測(cè)量依次進(jìn)行,兩次測(cè)量之間用淡水沖洗試 驗(yàn)箱,避免了傳統(tǒng) 密閉式測(cè)量方法導(dǎo)致的呼吸時(shí)內(nèi)產(chǎn)生缺氧狀態(tài)。 l 數(shù)據(jù)準(zhǔn)確,讀數(shù)穩(wěn)定,維護(hù)簡(jiǎn)單;· l 能長(zhǎng)期地自動(dòng)測(cè)量;· l 有不同大小呼吸室可以選擇,適宜于各種體積的水生動(dòng)物測(cè)量 l 所有部件統(tǒng)一安裝在堅(jiān)固的運(yùn)輸箱中,便于攜帶和存放。 |
測(cè)量方式
在實(shí)驗(yàn)過(guò)程中,水通過(guò)液流泵在動(dòng)物室和帶光學(xué)溶解氧探針的流通容器中循環(huán)。由數(shù)字控制單元(DCU)控制三通閥,軟件控制設(shè)定系統(tǒng)的循環(huán)或沖洗模式。
在循環(huán)模式下進(jìn)行呼吸測(cè)量,在沖洗模式下用水浴中的含氧水沖洗動(dòng)物室。循環(huán)-沖洗-循環(huán),重復(fù)運(yùn)行,直到實(shí)驗(yàn)結(jié)束。沖洗和循環(huán)階段的時(shí)間由用戶在軟件中選擇。
用于小樣本的mini-AQUA
對(duì)于小型魚(yú)類和昆蟲(chóng)幼蟲(chóng)(~1g)的水生呼吸測(cè)量,Qubit提供了mini-AQUA套裝。改良版的Q-Box Aqua帶有小型水生呼吸室(9ml)和用于更小系統(tǒng)體積的組件。還包括一個(gè)微型室(體積為1.23ml),用于測(cè)量小于0.5g的幼蟲(chóng)、卵和幼魚(yú)等非常小的樣品的耗氧率VO2。在測(cè)量期間,微型室采用間歇停止流動(dòng)模式,并將溶解氧探針直接放置在該室中,從而提高系統(tǒng)的靈敏度和分辨率。
v 4個(gè)數(shù)據(jù)通道
v 可變的數(shù)據(jù)采集速率
v 圖表、表格和儀表顯示
v 分析功能套件:
(統(tǒng)計(jì)、曲線擬合、積分等)
v 可輸入數(shù)據(jù)控制設(shè)備。
v 可生成多頁(yè)報(bào)告
v 數(shù)據(jù)回放功能
v 可輸入和編輯簡(jiǎn)單公式
v 自動(dòng)多種數(shù)學(xué)函數(shù)
v 動(dòng)態(tài)自動(dòng)計(jì)算
應(yīng)用領(lǐng)域
v 水生生物呼吸作用研究(測(cè)試溫度、缺氧、養(yǎng)分、壓力、體型對(duì)呼吸作用影響);
v 脊椎動(dòng)物和無(wú)脊椎動(dòng)物研究
v 實(shí)驗(yàn)室或野外均可應(yīng)用
基本組件
v 光學(xué)DO探頭和水浴
v 小型呼吸測(cè)量室(140ml)
v 大呼吸測(cè)量室(660ml)
v 水浴用溫鹽探頭
v 液體泵(1 LPM)和三通閥
v 三通閥數(shù)字控制單元
v 4通道數(shù)據(jù)接口
v 數(shù)據(jù)采集軟件
v 壓力傳感器
v 堅(jiān)固的防風(fēng)雨外殼
v 可選電池組
技術(shù)參數(shù)
客戶評(píng)價(jià)
“We have been using the Q-Box AQUA respirometry package for a year and have been impressed with the quality and versatility of the system. Although designed for teaching, we have found it to be more than adequate for basic research applications on both fish and cephalopods. The mechanical systems are well designed and easily serviced and the software is intuitive and flexible enough for use in diverse applications. The technicians at Qubit are outstanding; they were able to modify the system to suit our particular needs and they have always been quick to respond when technical support was required. The fact that Qubit ships the system in a protective case was also a big plus for field work!”
form Dr. Tyson MacCormak, Biology Department, Mount Alison University, New Brunswick, Canada
“我們已經(jīng)使用Q-Box AQUA呼吸測(cè)量軟件包一年了,它的質(zhì)量和多功能性給我們留下了深刻的印象。雖然是為教學(xué)而設(shè)計(jì)的,但我們發(fā)現(xiàn)它對(duì)于魚(yú)類和頭足類的基礎(chǔ)研究應(yīng)用來(lái)說(shuō)已經(jīng)足夠了。機(jī)械系統(tǒng)設(shè)計(jì)精良,維修方便,軟件直觀靈活,適用于多種應(yīng)用。Qubit的技術(shù)人員非常出色;他們能夠修改系統(tǒng)以滿足我們的特殊需要,且技術(shù)支持響應(yīng)迅速。Qubit系統(tǒng)裝在一個(gè)保護(hù)箱里,非常有益于野外工作!
發(fā)表文獻(xiàn)
· Bessemer RA et al. (2015) Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology 9: 861-870
· Callaghan NI et al. (2016) Zinc oxide nanoparticles trigger cardiorespiratory stress and reduce aerobic scope in the white sucker, Catostomus commersonii. Nanoimpact 2:29-37
· Black MN et al. (2017) Environmentally relevant concentrations of amine-functionalized copper nanoparticles exhibit different mechanisms of bioactivity in Fundulus Heteroclitus in fresh and brackish water. Nanotoxicology 11:1070-1085
· Keogh C et al. (2017) The double edge to parasite escape: invasive host is less infected but more infectable. Ecology 98:2241-2247
· Rangel RE, Johnson DW (2018) Metabolic responses to temperature in a sedentary reef fish, the bluebanded goby (Lythrypnus dalli, Gilbert). Journal of Experimental Marine Biology and Ecology 501: 83-89
Lamarre SG et al. (2019) Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
產(chǎn)地:加拿大