主要功能
對自然水體中的藍藻、綠藻、硅/甲藻和隱藻自動分類（定性）
自動測量水樣中藍藻、綠藻、硅/甲藻和隱藻的葉綠素 a 含量（定量）和總?cè)~綠素 a 含量
一杯自然水樣，同時獲得藍藻、綠藻、硅/甲藻和隱藻的光合活性：
光合效率和光合速率（相對電子傳遞速率）
快速光曲線并進行擬合
藻類的潛在光合效率
藻類的光保護能力
藻類耐受強光的能力
用戶可做自己的參考光譜
測量參數(shù)
Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和葉綠素含量等
應用領域
主要用于水生生物學、水域生態(tài)學、海洋學、湖沼學、水質(zhì)預警、微藻生理學、微藻抗逆性等領域，對于了解自然水樣中藻類種群的動態(tài)變化、水華預警、野外水體中光合作用的時空變化、校正初級生產(chǎn)力的計算等有較大幫助。
特別適于浮游植物動力學研究和有害水華的早期預警。
主要技術參數(shù)
主控單元：金屬外殼，包含所有的光電元件及樣品測量室
測量光： LED，440 nm，480 nm，540 nm，590 nm 和 625 nm，5 波長脈沖調(diào)制測量光，2 檔強度設置，8 檔調(diào)制頻率設置， 3 檔測量光自動高頻設置
光化光：板載多波長 LED 陣列，440 nm，480 nm，540 nm，590 nm，625 nm 和 420-640 nm（白光），提供持續(xù)光化光，可達 1400 μmol m-2 s-1，快速動力學閃光高達 7000 μmol m-2 s-1，飽和脈沖可達 5000 μmol m-2 s-1
遠紅光：725 nm LED
信號檢測：基于 H-10720 光電傳感器模塊的光電倍增檢測器
標準檢測過濾器：> 650 nm 長通濾光片
高分辨率的光電倍增管，葉綠素濃度檢測限低至 0.1μg/L，適用野外采集的藻濃度很稀的樣品
升級技術特點
可提供 5 種波長的脈沖調(diào)制測量光和光化光
的板載 LED 陣列芯片技術
可實時進行四種藻的分類
可進行標準 PAM 測量及不同波長強光化光誘導的毫秒級熒光上升動力學分析
可測定光系統(tǒng) II 功能性捕光截面積
內(nèi)置自動測量程序，易于操作
PHYTO-PAM-II vs PHYTO-PAM
| 便攜式 PHYTO-PAM-II | PHYTO-PAM | |
| 5 種不同波長的測量光用于生物體內(nèi)不同類型的天線色素熒光的激發(fā) | 4 種不同波長的光 | |
| 4 種色素類型的在線分類 | 3 種色素類型的在線分類 | |
| 6 種波長的光化光 | 1 種波長的光化光 | |
| 可以分別測量綠藻，藍藻，硅/甲藻以及含有藻紅蛋白的有機體，如隱藻不同波長下 PSII 的活性 | 無該功能 | |
| 緊湊型設計 | 需要組裝 | |
| 增加了快速動力學操作模式，可通過強光化光脈沖，測量不同波長的 O- I1 熒光上升動力學曲線 | 無該功能 | |
| 通過測定不同光質(zhì)和光合生物色素復合體，獲得光系統(tǒng) II 功能性捕光截面積的信息即 σPSII | 無該功能 | |
| 通過 FluoRed 熒光標準將參考光譜校準標準化 | 無該功能 | |
| 獲得的參考光譜可在不同設備及用戶間互換使用 | 參考光譜不能互換 |
選購指南
一、實驗室測量基本款
系統(tǒng)組成：實驗室版主機，激發(fā)檢測單元，懸浮液的光學單元，球形光量子傳感器，工作臺，軟件等
注意：版 PHYTO-PAM-II 必須配置光量子傳感器，校準光強后參考光譜可在不同儀器間通用
 |
| 實驗室測量基本款 |
二、野外便攜緊湊款
系統(tǒng)組成：緊湊型主機，球形光量子傳感器，軟件等
注意：版 PHYTO-PAM-II 必須配置光量子傳感器，校準光強后參考光譜可在不同儀器間通用
 |
| 便攜式緊湊款 |
 |  |  |
| 五種測量光通道 | 區(qū)分四種藻類 | 通用型參考光譜 |
 |  |  |
測量慢速動力學曲線 淬滅分析，暗弛豫分析 | 測量快速光曲線 光響應曲線 | 測量快速動力學曲線 分析PSII功能性捕光截面 |
三、其他可選附件
1，WATER-S：攪拌器（推薦選配），利用內(nèi)置電池驅(qū)動的馬達帶動攪拌棒旋轉(zhuǎn)，對樣品杯里的懸浮液進行攪拌。需配置攪拌棒 WATER-R。
2，WATER-R：攪拌棒（標配10根）
產(chǎn)地：德國WALZ
參考文獻
數(shù)據(jù)來源：光合作用文獻 Endnote 數(shù)據(jù)庫，更新至 2016 年 9 月，文獻數(shù)量超過 6000 篇
原始數(shù)據(jù)來源：Google Scholar
Biessy, L., et al. (2016). "Measuring the influence of nutrients and river water on the photosynthetic efficiency of Didymosphenia geminata using pulse amplitude modulated fluorometry." Diatom Research: 1-12.
Bondar-Kunze, E., et al. (2016). "Antagonistic and synergistic effects on a stream periphyton community under the influence of pulsed flow velocity increase and nutrient enrichment." Science of The Total Environment 573: 594-602.
Callieri, C., et al. (2016). "Grazing-induced Synechococcus microcolony formation: experimental insights from two freshwater phylotypes." FEMS microbiology ecology.
Cassell, R. T., et al. (2015). "Brevetoxin, the Dinoflagellate Neurotoxin, Localizes to Thylakoid Membranes and Interacts with the Light‐Harvesting Complex II (LHCII) of Photosystem II." ChemBioChem 16(7): 1060-1067.
Cecchi, P., et al. (2016). "Water flux management and phytoplankton communities in a Mediterranean coastal lagoon. Part II: Mixotrophy of dinoflagellates as an adaptive strategy?" Marine pollution bulletin.
Chen, C., et al. (2016). "Growth, physiochemical and antioxidant responses of overwintering benthic cyanobacteria to hydrogen peroxide." Environmental Pollution.
Chen, J.-W., et al. (2016). "Identification of a malonyl CoA-acyl carrier protein transacylase and its regulatory role in fatty acid biosynthesis in oleaginous microalga Nannochloropsis oceanica." Biotechnology and Applied Biochemistry: n/a-n/a.
Deng, X.-Y., et al. (2016). "Growth inhibition and oxidative stress induced by 1-octyl-3-methylimidazolium bromide on the marine diatom Skeletonema costatum." Ecotoxicology and Environmental Safety 132: 170-177.
Ding, Y., et al. (2016). "Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition." Chinese Journal of Oceanology and Limnology: 1-11.
Fabre, A., et al. (2016). "South American PSP toxin-producing Cylindrospermopsis raciborskii (Cyanobacteria) decreases clearance rates of cladocerans more than copepods." Hydrobiologia: 1-9.
Frenken, T., et al. (2016). "Warming accelerates termination of a phytoplankton spring bloom by fungal parasites." Global change biology 22(1): 299-309.
Huang, Y., et al. (2016). "Sinking loss should be taken into account while studying the dynamics of Microcystis under light-availability control." Journal of hazardous materials 314: 270-276.
Li, J. and X. Sun (2016). "Effects of different phosphorus concentrations and N/P ratios on the growth and photosynthetic characteristics of Skeletonema costatum and Prorocentrum donghaiense." Chinese Journal of Oceanology and Limnology: 1-15.
Li, S., et al. (2016). "Effects of nitrogen and phosphorus enrichment on growth and photosynthetic assimilation of carbon in a green tide-forming species (Ulva prolifera) in the Yellow Sea." Hydrobiologia: 1-11.
Liu, F., et al. (2016). "Development of an algal treatment system for Se removal: Effects of light regimes, nutrients, sulfate and hypersalinity." Chemosphere 164: 372-378.
Saeck, E. A., et al. (2016). "Nitrogen response of natural phytoplankton communities: a new indicator based on photosynthetic efficiency Fv/Fm." MARINE ECOLOGY PROGRESS SERIES 552: 81-92.
Schmitt-Jansen, M., et al. (2016). "Induced community tolerance of periphyton towards combined salt and toxic stress." Freshwater Biology: n/a-n/a.
Wang, C., et al. (2016). "Effects of iron on growth, antioxidant enzyme activity, bound extracellular polymeric substances and microcystin production of Microcystis aeruginosa FACHB-905." Ecotoxicology and Environmental Safety 132: 231-239.
Wang, X., et al. (2016). "Effects of Dracontomelon duperreanum defoliation extract on Microcystis aeruginosa: physiological and morphological aspects." Environmental Science and Pollution Research: 1-10.
Wang, Y., et al. (2016). "Contrasting response of a plankton community to two filter-feeding fish and their feces: An in situ enclosure experiment." Aquaculture.
Xu, J., et al. (2016). "The role of coccoliths in protecting Emiliania huxleyi against stressful light and UV radiation." Biogeosciences 13(16): 4637-4643.
Xu, J., et al. (2016). "Changes in growth, photosynthesis and chlorophyll fluorescence in the freshwater dinoflagellate Peridinium umbonatum (Peridiniales, Pyrrophyta) in response to different temperatures." Phycologia 55(4): 469-477.
Zhang, Y.-J., et al. (2015). "Proteomic analysis provides new insights into the adaptive response of a dinoflagellate Prorocentrum donghaiense to changing ambient nitrogen." Plant, Cell & Environment 38(10): 2128-2142.
Zhou, J., et al. (2016). "Incident Ultraviolet Irradiances Influence Physiology, Development and Settlement of Larva in the Coral Pocillopora damicornis." Photochemistry and Photobiology: n/a-n/a.
Zhou, Q., et al. (2016). "Nutrient removal by Chlorella vulgaris F1068 under cetyltrimethyl ammonium bromide induced hormesis." Environmental Science and Pollution Research: 1-11.
