多功能植物光合表型成像測(cè)量系統(tǒng)PlantExplorer采用創(chuàng)新的多光譜葉綠素?zé)晒?可見(jiàn)光成像技術(shù)，利用的LED技術(shù)、CCD技術(shù)、通信技術(shù)，實(shí)現(xiàn)了對(duì)植物表型的創(chuàng)新測(cè)量，可以在獲取RGB成像、葉綠素成像、花青素成像的同時(shí)，獲取葉綠素?zé)晒獬上瘢ǔ上衩娣e53cm x 53cm）。系統(tǒng)包括帶光學(xué)濾光輪的CCD成像系統(tǒng)、聚焦系統(tǒng)、嵌入式高亮度紅光LED、光譜白光LED、多光譜LED、嵌入式電腦和觸摸屏。由于采用一個(gè)CCD加濾光輪的組合，使得能夠在像素水平上進(jìn)行圖像疊加計(jì)算。

多功能植物光合表型成像測(cè)量系統(tǒng)PlantExplorer包括三個(gè)版本：標(biāo)準(zhǔn)版PlantExplorer、高級(jí)版本PlantExplorer^Pro和適合高達(dá)120cm植物的版本PlantExplorer^Pro+。三個(gè)版本都可以選配GFP和/或RFP成像模塊（需在購(gòu)買(mǎi)時(shí)指出，不可后續(xù)升級(jí)），其中PlantExplorer可以在購(gòu)買(mǎi)后，再后續(xù)升級(jí)成PlantExplorer^Pro。

功能特性

• 創(chuàng)新的多功能植物光合表型平臺(tái)
• 可見(jiàn)光成像+多光譜成像+葉綠素?zé)晒猓ㄕ{(diào)制和非調(diào)制）成像
• 同一個(gè)相機(jī)采集所有成像
• 全自動(dòng)馬達(dá)聚焦系統(tǒng)，帶全景和微距聚焦程序
• 出色的高清相機(jī)（1.3 M pixel）測(cè)量葉綠素?zé)晒?/li>
• 高信噪比葉綠素?zé)晒獬上?/li>
• 高質(zhì)量10 Mp鏡頭，帶光譜可見(jiàn)光和近紅外涂層
• 無(wú)可見(jiàn)鏡頭畸變，無(wú)需圖像校正
• 濾光片可提供10個(gè)濾光片位置
• 大景深設(shè)計(jì)
• 成像范圍53 x 53cm
• 可進(jìn)行多光譜測(cè)量，精確獲知葉綠素?zé)晒?、葉綠素、花青素和R/G/B圖像每個(gè)像素的變化
• 自動(dòng)計(jì)算熒光參數(shù)和表型參數(shù)
• 可設(shè)置進(jìn)行延時(shí)成像測(cè)量
• 嵌入式電腦進(jìn)行精確的成像、時(shí)間控制、光強(qiáng)控制和數(shù)據(jù)存儲(chǔ)
• 系統(tǒng)配置觸摸屏顯示器
• 功能強(qiáng)大的控制和分析軟件

選購(gòu)指南

主要技術(shù)參數(shù)

• 相機(jī)傳感器類(lèi)型：CCD
• 相機(jī)分辨率：130萬(wàn)像素
• 圖像獲取時(shí)間：?jiǎn)螐埲~綠素?zé)晒鈭D像20-1 000 us
• 圖像格式：16位RAW格式
• 光譜范圍：350～1000 nm
• 激發(fā)光強(qiáng)度：25cm處，1500-6000 umol m-2 s-1；60cm處，800-3500 umol m-2 s-1。強(qiáng)度可調(diào)。
• 光化光強(qiáng)度： 60cm處，100-600 umol m-2 s-1。強(qiáng)度可調(diào)。
• 光學(xué)濾光片（適用于多光譜版）：6種高質(zhì)量光學(xué)干涉濾光片，包括熒光、紅光、綠光、藍(lán)光、花青素和近紅外濾光片
• 成像面積：53 x 53 cm
• 成像和計(jì)算的參數(shù)：Fo成像、Fm成像、Ft成像、Ft=5min成像、Fm’成像、Fv/Fm成像、Fq’成像、ΦPSII成像、ΦRO成像、NPQ100成像、qN成像、qP成像、Rfd100成像、 NDVI成像、RNIR成像、RChl成像.、RAnth成像、RRed成像、RGreen成像、RBlue成像、葉綠素指數(shù)成像、花青素指數(shù)成像和可見(jiàn)光成像，能夠自動(dòng)計(jì)算投影葉面積、Fv/Fm平均值、低于Fv/Fm的面積百分比、ΦPSII平均值、低于ΦPSII的面積百分比、NPQ100平均值、高于NPQ100的面積百分比、Rfd100平均值、低于Rfd100的面積百分比、平均RGB比值、特殊RGB比值的面積百分比、平均葉綠素指數(shù)、低于葉綠素指數(shù)的面積百分比、平均花青素指數(shù)、低于花青素指數(shù)的面積百分比等（具體參數(shù)取決于版本），以及凸包、最小外接圓、最小外接矩形等相關(guān)表型參數(shù)。

應(yīng)用舉例

利用PhenoVation光合表型成像技術(shù)發(fā)表的部分文獻(xiàn)

1. Casto A L, Schuhl H, Schneider D, et al. (2021) Analyzing chlorophyll fluorescence images in PlantCV. Earth and Space Science Open Archive:5. https://doi.org/10.1002/essoar..2
2. Wang L, Liu F, Hao X, et al. (2021) Identification of the QTL-allele System Underlying Two High-Throughput Physiological Traits in the Chinese Soybean Germplasm Population. Frontiers in Genetics, https://doi.org/10.3389/fgene.2021.600444
3. Farooq M, van Dijk A D J, Nijveen H, et al. (2021) Prior Biological Knowledge Improves Genomic Prediction of Growth-Related Traits in Arabidopsis thaliana. Frontiers in Genetics, 11:609117. doi: 10.3389/fgene.2020.609117
4. He Y, Li Y, Yao Y et al. (2021) Overexpression of watermelon m⁶A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression. Plant Physiology and Biochemistry, 168: 340-352.
5. Wang W, Liu D, Qin M et al. (2021) Effects of Supplemental Lighting on Potassium Transport and Fruit Coloring of Tomatoes Grown in Hydroponics. International Journal of Molecular Sciences, 22(5): 2687 https://doi.org/10.3390/ijms
6. Singh R R, Pajar J A, Audenaert K, et al. (2021) Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes. Frontiers in Plant Science, 12:713870. doi: 10.3389/fpls.2021.713870
7. Vidak M, Lazarevic B, Petek M, et al. (2021) Multispectral Assessment of Sweet Pepper (Capsicum annuum L.) Fruit Quality Affected by Calcite Nanoparticles. Biomolecules, 11(6), 832; https://doi.org/10.3390/biom
8. Lazarevic B, Satovic Z, Nimac A, et al. (2021) Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.). Frontiers in Plant Science, 12:629441. doi: 10.3389/fpls.2021.629441
9. Romero-Perez A, Ameye M, Audenaert K, et al. (2021) Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation in Arabidopsis thaliana After Pseudomonas syringae Infection. Frontiers in Plant Science, 12:692606. doi: 10.3389/fpls.2021.692606
10. Meng L, Mestdagh H, Ameye M, et al. (2021) Phenotypic variation of Botrytis cinerea Isolates is influenced by spectral light quality. Frontiers in Plant Science, 11:1233. doi: 10.3389/fpls.2020.01233
11. De Zutter N, Ameye M, Debode J, et al. (2021) Shifts in the rhizobiome during consecutive in planta enrichment for phosphate-solubilizing bacteria differentially affect maize P status. Microbial Biotechnology, doi:10.1111/1751-7915.13824
12. Stambuk P, Sikuten I, Preiner D, et al. (2021) Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging. Plants, 10, 661. https://doi.org/10.3390/plants
13. Tan J, de Zutter N, de Saeger S, et al. (2021) Presence of the Weakly Pathogenic Fusarium poae in the Fusarium Head Blight Disease Complex Hampers Biocontrol and Chemical Control of the Virulent Fusarium graminearum Pathogen. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.641890
14. Flood P, Theeuwen T, Schneeberger K, Keizer P, Kruijer W, et al. (2020) Reciprocal cybrids reveal how organellar genomes affect plant phenotypes. Nature Plants, 10.1038/s41477-019-0575-9ff. ffhal-v2f
15. Velivelli S L S, Czymmek K J, Li H, Shaw J B, Buchko G W, Shah D M. (2020) Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection. PNAS, DOI: 10.1073/pnas.2003526117
16. Bhatnagar N, Pandey S. (2020) Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants. Plant Physiology, DOI: https://doi.org/10.1104/pp.20.01309
17. Venneman J, Vandermeersch L, Walgraeve C et al. (2020) Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2020.544435
18. Tan J, Ameye M, Landschoot S et al. (2020) At the scene of the crime: New insights into the role of weakly pathogenic members of the fusarium head blight disease complex. Molecular Plant Pathology, DOI: 10.1111/mpp.12996
19. Prinzenberg A E, Campos-Dominguez L, Kruijer W, Harbinson J, Aarts M G M. (2020) Natural variation of photosynthetic efficiency in Arabidopsis thaliana accessions under low temperature conditions. Plant Cell & Environment, 1–14. https://doi.org/10.1111/pce.13811
20. Zhang H, Chen Y, Niu Y, Zhang X, Zhao J, Sun L, Wang H, Xiao J, Wang X. (2020) Characterization and fine mapping of a leaf yellowing mutant in common wheat. Plant Growth Regulation, https://doi.org/10.1007/s10725-020-00633-0
21. Jin X, Zarco-Tejada P, Schmidhalter U, Reynolds M P et al. (2020) High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms. IEEE Geoscience and Remote Sensing Magazine, DOI: 10.1109/MGRS.2020.2998816
22. Sheng X-G, Branca F, Zhao Z-Q et al. (2020) Identification of Black Rot Resistance in a Wild Brassica Species and Its Potential Transferability to Cauliflower. Argonomy, 10: 1400. doi:10.3390/agronomy
23. Pennisi G, Blasioli S, Cellini A, Maia L, Crepaldi A, Braschi I, Gianquinto G. (2019). Unraveling the Role of Red:Blue LED Lights on Resource Use Efficiency and Nutritional Properties of Indoor Grown Sweet Basil. Frontiers in plant science, 10, 305. doi:10.3389/fpls.2019.00305
24. Pennisi G, Orsini F, Blasioli S, Cellini A et al. (2019) Resource use efficiency of indoor lettuce (Lactuca sativa L.) c*tion as affected by red:blue ratio provided by LED lighting. Scientific Reports, 9, 14127
25. Van Es S W, van der Auweraert E B, Silveira S R, Angenent G C, van Dijk A D J, Immink R G H. (2019) Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination. The Plant Journal, doi: 10.1111/tpj.14326
26. Köhl J, Goossen-van de Geijn H, Groenenboom-de Haas L, et al. (2019) Stepwise screening of candidate antagonists for biological control of Blumeria graminis f. sp. tritici. Biological Control, 136: 104008
27. Mohd Nadzir M M, Vieira Lelis F M, Thapa B, Ali A, Visser R G F, van Heusden A W, van der Wolf J M. (2019) Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species. Plant Phathology, 68(1): 42-48
28. Sall K, Dekkers B J W, Nonogaki M, Katsuragawa Y, Koyari R, Hendrix D, Willems L A J, Bentsink L, Nonogaki H. (2019) DELAY OF GERMINATION  1‐LIKE  4 acts as an inducer of seed reserve accumulation. The Plant Journal, 100: 7-19.
29. Li H, Velivelli S L S, Shah D M. (2019) Antifungal Potency and Modes of Action of a Novel Olive Tree Defensin Against Closely Related Ascomycete Fungal Pathogens. Molecular Plant-Microbe Interactions. 32(12): 1646-1664.
30. Prinzenberg A E, Viquez-Zamora M, Harbinson J, Lindhout P, van Heusden S. (2018) Chlorophyll fluorescence imaging reveals genetic variationａnd loci for a photosynthetic trait in diploid potato. Physiologia Plantarum, 164: 163-175.
31. Van Rooijen R, Harbinson J, Aarts M G M. (2018) Photosynthetic response to increased irradiance correlates to variation in transcriptional response of lipid‐remodeling and heat‐shock genes. Plant Direct, 2(7): e00069
32. Van Bezouw R F H M, Keurentjes J J B, Harbinson J, Aarts M G. (2018) Converging phenomics and genomics to study natural variation in plant photosynthetic efficiency. Plant Journal, 97(1): 112-133.
33. Domazakis E, Wouters D, Visser R G F, Kamoun S, Joosten M H A J, Vleeshouwers V G A A. (2018) The ELR-SOBIR1 Complex Functions as a Two-Component Receptor-Like Kinase to Mount Defense Against Phytophthora infestans. Molecular Plant-Microbe Interactions, 31(8): 795-802.
34. Bazakos C, Hanemian M, Trontin C, Jimenez-Gomez J M, Loudet O. (2017) New Strategies and Tools in Quantitative Genetics: How to Go from the Phenotype to the Genotype. Annual Review of Plant Biology, 68:435-455
35. Van Rooijen R, Kruijer W, Boesten R, van Eeuwijk F A, Harbinson J, Aarts M G M. (2017) Natural variation of YELLOW SEEDLING1 affects photosynthetic acclimation of Arabidopsis thaliana. Nature Communications, 8: 1421
36. Flood P J, Kruijer W, Schnabel S K, van der Schoor R, Jalink H, Snel J F H, Harbinson J, Aarts M G M. (2016) Phenomics for photosynthesis, growth and reflectance in Arabidopsis thaliana reveals circadian and long-term fluctuations in heritability. Plant Methods, 12: 14. https://doi.org/10.1186/s13007-016-0113-y
37. Mancarella S, Orsini F, van Oosten M J, SAnoubar R, Stanghellini C, Kondo S, Gianquinto G, Maggio A. (2016) Leaf sodium accumulation facilitates salt stress adaptation and preserves photosystem functionality in salt stressed Ocimum basilicum. Environmental and Experimental Botany, 130: 162-173.
38. Virlet N, Sabermanesh K, Sadeghi-Tehran P, Hawkesford M J. (2016) Field Scanalyzer: An automated robotic field phenotyping platform for detailed crop monitoring. Functional Plant Biology, 44(1): 143-153.
39. Gorbe Sanchez E, Heuvelink E, de Gelder A, Stanghellini C. (2015) New Non-invasive Tools for Early Plant Stress Detection. Procedia Environmental Sciences, 29: 249-250.
40. Kastelein P, Krijger M, Czajkowski R, van der Zouwen P S, van der Schoor R, Jalink H, van der Wolf J M. (2014) Development of Xanthomonas fragariae populations and disease progression in strawberry plants after spray‐inoculation of leaves. Plant Pathology, 63(2): 255-263.
41. Harbinson J, Prinzenberg A E, Kruijer W, Aarts M G M. (2012) High throughput screening with chlorophyll ?uorescence imaging and its use in crop improvement. Current Opinion in Biotechnology, 23:221