移動式大型植物光合表型測量系統(tǒng)CropReporter采用一體化“可見光成像+葉綠素?zé)晒獬上?多光譜成像”技術(shù)對冠層尺寸60cm x 74cm范圍內(nèi)的植物進行深入的光合表型和形態(tài)結(jié)構(gòu)表型測量分析。系統(tǒng)成像單元配置白光LED和405 nm、460 nm、520 nm、660 nm、720 nm的LED，成像單元除了能夠測量數(shù)十個光合熒光參數(shù)外，還能夠測量葉綠素成像、花青素成像、NDVI和可見光表型等（軟件直接生成）。

系統(tǒng)配備4個輪子，樣品室前后門可拉伸升降，可以方便的實現(xiàn)溫室內(nèi)基于“Sensor-to-Plant”模式的人工輔助高通量測量。

功能特性

• 標(biāo)準(zhǔn)版成像面積74 x 60cm，可以移動，適合在溫室內(nèi)測量
• 可定制成像面積達200 x 200cm 
• 在百萬像素水平上實現(xiàn)對熒光誘導(dǎo)曲線Fo、FI和FP相的測量
• 全部采用LED光源來提供紅外、遠紅外、白光和近紅外
• 儀器內(nèi)置電腦用于成像測量、光照控制和數(shù)據(jù)存儲
• 實現(xiàn)多光譜測量，在測量光合作用之外，獲取葉綠素指數(shù)、花青素指數(shù)、NDVI、紅外成像和RGB成像。
• 對熒光成像和多光譜成像進行逐個像素分析
• 無二的大型植物測量技術(shù)，成像面積從74 x 60 cm到200 x 200 cm 
• 定制化設(shè)計，可以頂部成像，也可以側(cè)面成像，甚至頂部和側(cè)面都成像
• 可以集成到基于傳送系統(tǒng)的高通量植物表型平臺中，進行高通量的光合表型測量
• 大景深成像技術(shù)

典型應(yīng)用

主要技術(shù)參數(shù)

• 相機傳感器類型：CCD
• 相機分辨率：1.3M像素
• 圖像格式：16位RAW格式、JPG格式
• 光譜范圍：350～950 nm
• 光學(xué)濾光片：6種高質(zhì)量光學(xué)干涉濾光片，包括熒光、紅光、綠光、藍光、花青素和近紅外濾光片
• 多光譜和彩色圖像光源：白色LED和和405 nm、460 nm、520 nm、660 nm、720 nm的LED
• LED控制：恒流電源控制LED強度，不采用脈沖調(diào)制控制
• 成像和計算的參數(shù)：Fo成像、Fm成像、Ft成像、Ft=5min成像、Fm?成像、Fv/Fm成像、Fq?成像、ΦPSII成像、ΦRO成像、NPQ100成像、qN成像、qP成像、Rfd100成像、 NDVI成像、RNIR成像、RChl成像.、RAnth成像、RRed成像、RGreen成像、RBlue成像、葉綠素指數(shù)成像、花青素指數(shù)成像和可見光成像，能夠自動計算投影葉面積、Fv/Fm平均值、低于Fv/Fm的面積百分比、ΦPSII平均值、低于ΦPSII的面積百分比、NPQ100平均值、高于NPQ100的面積百分比、Rfd100平均值、低于Rfd100的面積百分比、平均RGB比值、特殊RGB比值的面積百分比、平均葉綠素指數(shù)、低于葉綠素指數(shù)的面積百分比、平均花青素指數(shù)、低于花青素指數(shù)的面積百分比等（具體參數(shù)取決于版本） ，以及凸包、最小外接圓、最小外接矩形等相關(guān)表型參數(shù)。 

利用PhenoVation光合表型成像技術(shù)發(fā)表的部分文獻

1. Casto A L, Schuhl H, Schneider D, et al. (2021) Analyzing chlorophyll fluorescence images in PlantCV. Earth and Space Science Open Archive:5. https://doi.org/10.1002/essoar..2
2. Wang L, Liu F, Hao X, et al. (2021) Identification of the QTL-allele System Underlying Two High-Throughput Physiological Traits in the Chinese Soybean Germplasm Population. Frontiers in Genetics, https://doi.org/10.3389/fgene.2021.600444
3. Farooq M, van Dijk A D J, Nijveen H, et al. (2021) Prior Biological Knowledge Improves Genomic Prediction of Growth-Related Traits in Arabidopsis thaliana. Frontiers in Genetics, 11:609117. doi: 10.3389/fgene.2020.609117
4. He Y, Li Y, Yao Y et al. (2021) Overexpression of watermelon m⁶A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression. Plant Physiology and Biochemistry, 168: 340-352.
5. Wang W, Liu D, Qin M et al. (2021) Effects of Supplemental Lighting on Potassium Transport and Fruit Coloring of Tomatoes Grown in Hydroponics. International Journal of Molecular Sciences, 22(5): 2687 https://doi.org/10.3390/ijms
6. Singh R R, Pajar J A, Audenaert K, et al. (2021) Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes. Frontiers in Plant Science, 12:713870. doi: 10.3389/fpls.2021.713870
7. Vidak M, Lazarevic B, Petek M, et al. (2021) Multispectral Assessment of Sweet Pepper (Capsicum annuum L.) Fruit Quality Affected by Calcite Nanoparticles. Biomolecules, 11(6), 832; https://doi.org/10.3390/biom
8. Lazarevic B, Satovic Z, Nimac A, et al. (2021) Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.). Frontiers in Plant Science, 12:629441. doi: 10.3389/fpls.2021.629441
9. Romero-Perez A, Ameye M, Audenaert K, et al. (2021) Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation in Arabidopsis thaliana After Pseudomonas syringae Infection. Frontiers in Plant Science, 12:692606. doi: 10.3389/fpls.2021.692606
10. Meng L, Mestdagh H, Ameye M, et al. (2021) Phenotypic variation of Botrytis cinerea Isolates is influenced by spectral light quality. Frontiers in Plant Science, 11:1233. doi: 10.3389/fpls.2020.01233
11. De Zutter N, Ameye M, Debode J, et al. (2021) Shifts in the rhizobiome during consecutive in planta enrichment for phosphate-solubilizing bacteria differentially affect maize P status. Microbial Biotechnology, doi:10.1111/1751-7915.13824
12. Stambuk P, Sikuten I, Preiner D, et al. (2021) Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging. Plants, 10, 661. https://doi.org/10.3390/plants
13. Tan J, de Zutter N, de Saeger S, et al. (2021) Presence of the Weakly Pathogenic Fusarium poae in the Fusarium Head Blight Disease Complex Hampers Biocontrol and Chemical Control of the Virulent Fusarium graminearum Pathogen. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.641890
14. Flood P, Theeuwen T, Schneeberger K, Keizer P, Kruijer W, et al. (2020) Reciprocal cybrids reveal how organellar genomes affect plant phenotypes. Nature Plants, 10.1038/s41477-019-0575-9ff. ffhal-v2f
15. Velivelli S L S, Czymmek K J, Li H, Shaw J B, Buchko G W, Shah D M. (2020) Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection. PNAS, DOI: 10.1073/pnas.2003526117
16. Bhatnagar N, Pandey S. (2020) Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants. Plant Physiology, DOI: https://doi.org/10.1104/pp.20.01309
17. Venneman J, Vandermeersch L, Walgraeve C et al. (2020) Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2020.544435
18. Tan J, Ameye M, Landschoot S et al. (2020) At the scene of the crime: New insights into the role of weakly pathogenic members of the fusarium head blight disease complex. Molecular Plant Pathology, DOI: 10.1111/mpp.12996
19. Prinzenberg A E, Campos-Dominguez L, Kruijer W, Harbinson J, Aarts M G M. (2020) Natural variation of photosynthetic efficiency in Arabidopsis thaliana accessions under low temperature conditions. Plant Cell & Environment, 1–14. https://doi.org/10.1111/pce.13811
20. Zhang H, Chen Y, Niu Y, Zhang X, Zhao J, Sun L, Wang H, Xiao J, Wang X. (2020) Characterization and fine mapping of a leaf yellowing mutant in common wheat. Plant Growth Regulation, https://doi.org/10.1007/s10725-020-00633-0
21. Jin X, Zarco-Tejada P, Schmidhalter U, Reynolds M P et al. (2020) High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms. IEEE Geoscience and Remote Sensing Magazine, DOI: 10.1109/MGRS.2020.2998816
22. Sheng X-G, Branca F, Zhao Z-Q et al. (2020) Identification of Black Rot Resistance in a Wild Brassica Species and Its Potential Transferability to Cauliflower. Argonomy, 10: 1400. doi:10.3390/agronomy
23. Pennisi G, Blasioli S, Cellini A, Maia L, Crepaldi A, Braschi I, Gianquinto G. (2019). Unraveling the Role of Red:Blue LED Lights on Resource Use Efficiency and Nutritional Properties of Indoor Grown Sweet Basil. Frontiers in plant science, 10, 305. doi:10.3389/fpls.2019.00305
24. Pennisi G, Orsini F, Blasioli S, Cellini A et al. (2019) Resource use efficiency of indoor lettuce (Lactuca sativa L.) c*tion as affected by red:blue ratio provided by LED lighting. Scientific Reports, 9, 14127
25. Van Es S W, van der Auweraert E B, Silveira S R, Angenent G C, van Dijk A D J, Immink R G H. (2019) Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination. The Plant Journal, doi: 10.1111/tpj.14326
26. Köhl J, Goossen-van de Geijn H, Groenenboom-de Haas L, et al. (2019) Stepwise screening of candidate antagonists for biological control of Blumeria graminis f. sp. tritici. Biological Control, 136: 104008
27. Mohd Nadzir M M, Vieira Lelis F M, Thapa B, Ali A, Visser R G F, van Heusden A W, van der Wolf J M. (2019) Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species. Plant Phathology, 68(1): 42-48
28. Sall K, Dekkers B J W, Nonogaki M, Katsuragawa Y, Koyari R, Hendrix D, Willems L A J, Bentsink L, Nonogaki H. (2019) DELAY OF GERMINATION  1‐LIKE  4 acts as an inducer of seed reserve accumulation. The Plant Journal, 100: 7-19.
29. Li H, Velivelli S L S, Shah D M. (2019) Antifungal Potency and Modes of Action of a Novel Olive Tree Defensin Against Closely Related Ascomycete Fungal Pathogens. Molecular Plant-Microbe Interactions. 32(12): 1646-1664.
30. Prinzenberg A E, Viquez-Zamora M, Harbinson J, Lindhout P, van Heusden S. (2018) Chlorophyll fluorescence imaging reveals genetic variationａnd loci for a photosynthetic trait in diploid potato. Physiologia Plantarum, 164: 163-175.
31. Van Rooijen R, Harbinson J, Aarts M G M. (2018) Photosynthetic response to increased irradiance correlates to variation in transcriptional response of lipid‐remodeling and heat‐shock genes. Plant Direct, 2(7): e00069
32. Van Bezouw R F H M, Keurentjes J J B, Harbinson J, Aarts M G. (2018) Converging phenomics and genomics to study natural variation in plant photosynthetic efficiency. Plant Journal, 97(1): 112-133.
33. Domazakis E, Wouters D, Visser R G F, Kamoun S, Joosten M H A J, Vleeshouwers V G A A. (2018) The ELR-SOBIR1 Complex Functions as a Two-Component Receptor-Like Kinase to Mount Defense Against Phytophthora infestans. Molecular Plant-Microbe Interactions, 31(8): 795-802.
34. Bazakos C, Hanemian M, Trontin C, Jimenez-Gomez J M, Loudet O. (2017) New Strategies and Tools in Quantitative Genetics: How to Go from the Phenotype to the Genotype. Annual Review of Plant Biology, 68:435-455
35. Van Rooijen R, Kruijer W, Boesten R, van Eeuwijk F A, Harbinson J, Aarts M G M. (2017) Natural variation of YELLOW SEEDLING1 affects photosynthetic acclimation of Arabidopsis thaliana. Nature Communications, 8: 1421
36. Flood P J, Kruijer W, Schnabel S K, van der Schoor R, Jalink H, Snel J F H, Harbinson J, Aarts M G M. (2016) Phenomics for photosynthesis, growth and reflectance in Arabidopsis thaliana reveals circadian and long-term fluctuations in heritability. Plant Methods, 12: 14. https://doi.org/10.1186/s13007-016-0113-y
37. Mancarella S, Orsini F, van Oosten M J, SAnoubar R, Stanghellini C, Kondo S, Gianquinto G, Maggio A. (2016) Leaf sodium accumulation facilitates salt stress adaptation and preserves photosystem functionality in salt stressed Ocimum basilicum. Environmental and Experimental Botany, 130: 162-173.
38. Virlet N, Sabermanesh K, Sadeghi-Tehran P, Hawkesford M J. (2016) Field Scanalyzer: An automated robotic field phenotyping platform for detailed crop monitoring. Functional Plant Biology, 44(1): 143-153.
39. Gorbe Sanchez E, Heuvelink E, de Gelder A, Stanghellini C. (2015) New Non-invasive Tools for Early Plant Stress Detection. Procedia Environmental Sciences, 29: 249-250.
40. Kastelein P, Krijger M, Czajkowski R, van der Zouwen P S, van der Schoor R, Jalink H, van der Wolf J M. (2014) Development of Xanthomonas fragariae populations and disease progression in strawberry plants after spray‐inoculation of leaves. Plant Pathology, 63(2): 255-263.
41. Harbinson J, Prinzenberg A E, Kruijer W, Aarts M G M. (2012) High throughput screening with chlorophyll ?uorescence imaging and its use in crop improvement. Current Opinion in Biotechnology, 23:221