PerkinElmer’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.
The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
Features:
- Fully automatable and miniaturizable to 1536
- Enables reduction in reagents and consumable costs
- High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
- Robust assay (Z′, CV values, solvent resistance)
- Reduces the number of false positives from autofluorescent compounds in screening libraries
- Option to work with cells in suspension to increase throughput
- Amenable for use with calcium coupled ion channels and tyrosine kinase receptors
PerkinElmer’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.
規(guī)格
| 檢測(cè)目標(biāo)類 | GPCR |
|---|---|
| 檢測(cè)目標(biāo)類型 | Cell line |
| 檢測(cè)驗(yàn)證 | Binding, Calcium Luminescence |
| G-Alpha天然受體 | Gi/Go |
| 宿主細(xì)胞 | CHO-K1 |
| 產(chǎn)品品牌名稱 | AequoScreen |
| 受體類型 | 5-Hydroxytryptamine (Serotonin) |
| 第二信使釋放 | Calcium flux |
| 運(yùn)輸條件 | 干冰 |
| 特殊訂購(gòu)信息 | Requires accepted Terms and Conditions. Additional agreement needed for commercial use. |
| 治療領(lǐng)域 | Central Nervous System, Cardiovascular, Inflammation, Metabolic, Gastrointestinal |
| 產(chǎn)品尺寸 | 5 million cells |
