Thorlabs Bergamo® II系列多光子顯微鏡
遵循顯微鏡設(shè)計(jì) bi 須 符合樣品要求的原則，創(chuàng)建了一套 wan 全 模塊化的成像平臺(tái)，可以滿(mǎn)足多種實(shí)驗(yàn)需求。與傳統(tǒng)的研究技術(shù)(例如使用微電極)相比，我們的多光子成像系統(tǒng)能夠以更快速度、更大深度和更高強(qiáng)度同時(shí)讀出和操縱神經(jīng)元群。
Thorlabs Bergamo® II系列多光子顯微鏡
遵循顯微鏡設(shè)計(jì) bi 須 符合樣品要求的原則，創(chuàng)建了一套 wan 全 模塊化的成像平臺(tái)，可以滿(mǎn)足多種實(shí)驗(yàn)需求。與傳統(tǒng)的研究技術(shù)(例如使用微電極)相比，我們的多光子成像系統(tǒng)能夠以更快速度、更大深度和更高強(qiáng)度同時(shí)讀出和操縱神經(jīng)元群。如下面的選項(xiàng)概覽部分和右側(cè)較下方的旋轉(zhuǎn)Bergamo顯微鏡的五軸運(yùn)動(dòng)視頻所示，我們開(kāi)發(fā)了一個(gè)旋轉(zhuǎn)主體，使顯微鏡可繞樣品旋轉(zhuǎn)。我們還提供各種剛性主體，其具有業(yè)界 ling,先 的7.74英寸高度，可圍繞物鏡提供較大的三維工作空間。
亮點(diǎn)
● 基于貝塞爾光束的快速體積成像
● 三光子成像
● 空間光調(diào)制器用于同時(shí)多點(diǎn)刺激
Thorlabs Bergamo® II系列多光子顯微鏡
基于貝塞爾光束的快速體積成像
Thorlabs與Howard Hughes yi,學(xué)院 以及Na Ji教授(加利福尼亞大學(xué)伯克利分校)合作，為我們的Bergamo®多光子激光掃描顯微鏡提供了貝塞爾光束模塊。神經(jīng)元活動(dòng)的體內(nèi)體積成像需要亞微米級(jí)空間分辨率和毫秒級(jí)時(shí)間分辨率。傳統(tǒng)方法通過(guò)連續(xù)掃描衍射 ji 限 高斯光束以創(chuàng)建3D圖像，而基于貝塞爾光束的多光子成像依靠軸向延伸的焦點(diǎn)來(lái)捕獲體積圖像。激發(fā)光束的擴(kuò)展景深可創(chuàng)建3D體積的2D投影，從而 you 效 地將2D幀速率轉(zhuǎn)換為3D體積速率。
單次貝塞爾掃描(左)可捕獲與堆疊45個(gè)光學(xué)切片(右)而創(chuàng)建的高斯體積掃描相同的結(jié)構(gòu)信息，從而將總掃描時(shí)間減少45倍。圖像顯示了300 μm x 300 μm范圍內(nèi)掃描的大腦切片。高斯堆疊的掃描深度由比例尺指示。樣本由加州大學(xué)伯克利分校物理系Ji實(shí)驗(yàn)室的Qinrong Zhang博士和Matthew Jacobs提供。
規(guī)格
| Laser Scanning | |||
|---|---|---|---|
| Scan Path Wavelength Range | 450 - 1100 nm, 680 - 1300 nm, or 800 - 1800 nm | ||
| Scan Paths | Resonant-Galvo-Galvo Scanner, Galvo-Resonant Scanners, Galvo-Galvo Scanners, or Spatial Light Modulator; Single or Dual Scan Paths | ||
| Scan Speed | 8 kHz Resonant-Galvo-Galvo or Galvo-Resonant | 2 fps at 4096 x 4096 Pixels 30 fps at 512 x 512 Pixels 400 fps at 512 x 32 Pixels | |
| 12 kHz Resonant-Galvo-Galvo or Galvo-Resonant | 4.4 fps at 2048 x 2048 Pixels 45 fps at 512 x 512 Pixels 600 fps at 512 x 32 Pixels | ||
| Galvo-Galvo | 3 fps at 512 x 512 Pixels 48 fps at 512 x 32 Pixels 70 fps at 32 x 32 Pixels Pixel Dwell Time: 0.4 to 20 µs | ||
| Galvo-Galvo Scan Modes | Imaging: Line, Polyline, Square, or Rectangle Non-Imaging: Circle, Ellipse, Polygon, or Point | ||
| Field of View | FN40 Equivalent to 40 mm Diagonal Square (Max) at the Intermediate Image Plane >2.8 mm x 2.8 mm at the Sample plane with a 10X Objective FN20 Equivalent to 20 mm Diagonal Square (Max) at the Intermediate Image Plane >1.4 mm x 1.4 mm at the Sample plane with a 10X Objective | ||
| Scan Zoom | 1X to 16X (Continuously Variable) | ||
| Scan Resolution | Up to 2048 x 2048 Pixels (Bi-Directional) [Up to 1168 x 1168 Pixels for 12 kHz Scanners] Up to 4096 x 4096 Pixels (Unidirectional) [Up to 2336 x 2336 Pixels for 12 kHz Scanners] | ||
| Compatible Objective Threadings | M34 x 1.0, M32 x 0.75, M25 x 0.75, and RMS | ||
| Spatial Light Modulator (SLM) | ||||
|---|---|---|---|---|
| Stimulation Area (with a 16X Objective) | 600 µm x 600 µm (X and Y) ±150 µm (Z) | |||
| Resolution | 1024 pixels x 1024 pixels | |||
| Maximum Pattern Refresh Rate | 4 ms | |||
| Multiphoton Signal Detection | ||
|---|---|---|
| Epi Detection | Up to Four Ultrasensitive GaAsP PMTs, Cooled or Non-Cooled | |
| Forward-Direction Detection | Two Ultrasensitive GaAsP PMTs | |
| Maximum of Four PMTs Controlled by the Software at a Given Time | ||
| Collection Optics | 8°, 10°, or 14° Collection Angle (Angles Quoted When Using an Objective with a 20 mm Entrance Pupil) Easy-to-Exchange Emission Filters and Dichroic Mirrors | |
| Confocal Imaging | ||
|---|---|---|
| Motorized Pinhole Wheel with 16 Round Pinholes from ?25 µm to ?2 mm Two to Four Laser Lines (488 nm Standard; Other Options Range from 405 nm to 660 nm) Standard Multialkali or High-Sensitivity GaAsP PMTs Easy-to-Exchange Emission Filters and Dichroic Mirrors |
| Widefield Viewing | ||
|---|---|---|
| Manual or Motorized Switching Between Scanning and Widefield Modes Illumination Provided via LED or Liquid Light Guide C-Mount Threads for Scientific Cameras |
| Transmitted Light Imaging | ||
|---|---|---|
| Differential Interference Contrast (DIC) or Dodt Gradient Contrast Widefield or Laser Scanned Illumination Provided by Visible and/or NIR LEDs Compatible with Air or Oil Immersion Condensers |
| Three-Photon Imaging | ||
|---|---|---|
| Scan Optics for 800 - 1800 nm Range Achieve Reduced Background Scatter for Greater Sensitivity in Deep-Tissue Imaging |
| Volume Imaging Using Bessel Beams | ||
|---|---|---|
| 3D Volumetric Functional Imaging at Video Frame Rates Enhanced Temporal Resolution for Studying Internal Systems at Cellular Lateral Resolution In Vivo |
| Translation | ||||
|---|---|---|---|---|
| Microscope Body Rotation (Rotating Bodies Only) | 0° to 90° or -45° to +45° Around Objective Focus 0.1° Encoder Resolution | |||
| Coarse Elevator Base Z (Rotating Bodies Only) | 5" (127 mm) Total Travel; 1 µm Encoder Resolution | |||
| Fine Microscope Body X and Y | 2" (50.8 mm) Total Travel; 0.5 µm Encoder Resolution | |||
| Fine Microscope Arm Z | 1" (25.4 mm) Total Travel; 0.1 µm Encoder Resolution | |||
| Fine Z Focus | ||||
|---|---|---|---|---|
| Piezo Objective Scanner | Open Loop: 600 µm ± 10% Travel Range; 1 nm Resolution Closed Loop: 450 µm Travel Range; 3 nm Resolution | |||
| Vibrationless Remote Focus | 16 Discrete Steps ~400 µm Travel Range with a 10X Objective ~160 µm Travel Range with a 16X Objective | |||
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